Summary: Beta defensin
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Beta defensin Edit Wikipedia article
Defensins are 2-6 kDa, cationic, microbicidal peptides active against many Gram-negative and Gram-positive bacteria, fungi, and enveloped viruses, containing three pairs of intramolecular disulfide bonds. On the basis of their size and pattern of disulfide bonding, mammalian defensins are classified into alpha, beta and theta categories. Every mammalian species explored thus far has beta-defensins. In cows, as many as 13 beta-defensins exist in neutrophils. However, in other species, beta-defensins are more often produced by epithelial cells lining various organs (e.g. the epidermis, bronchial tree and genitourinary tract.
Human, rabbit and guinea-pig beta-defensins, as well as human beta-defensin-2 (hBD2), induce the activation and degranulation of mast cells, resulting in the release of histamine and prostaglandin D2.
 β-defensins and genes
β-defensins are coding for genes which impact the function of the innate immune system. These genes are responsible for production of antimicrobial polypeptides found in white blood cells such as macrophages, granulocytes and NK-cells, β-defensins are also found in epithelial cells. Single-nucleotide polymorphisms (SNPs) are found in genes coding for β-defensins. The presences of SNPs are lower in the coding regions compared to non-coding regions. The appearance of SNPs in the coding region will highly likely affecting the resistance against infections through changes in the protein sequences which will give rise to different biological functions.
Receptors such as Toll-like receptors (TLR) and nod-like receptors (NLR) will activate the immune system by binding of ligands such as lipopolysaccharides and peptidoglycan. Toll-like receptors are expressed in antigen presenting cells (APCs) such as dendritic cells, B-lymphocytes and macrophages. When the receptors are activated a cascade reaction will take place and substances such as cytokines and antimicrobial peptides will be released.
β-defensins are cationic and can therefore interact with the membrane of invading microbes, which are negative due to lipopolysaccharides (LPS) and lipoteichoic acid (LTA) found in the cell membrane. The peptides have higher affinity to the binding site compared to Ca2+ and Mg2+ ions. The peptides will therefore exchange place with those ions, thus affecting the stability of the membrane. The peptides have a greater size compared with the ions which cause changes in the membrane structure. Due to changes in the electric potential, peptides will pass across the membrane and thus aggregate into dimers. Pore complex will be created as a result of breaking the hydrogen bonds between the amino acids in the terminal end of the strands connecting defensins monomers. Formation of pore complex will cause membrane depolarization and cell lysis.
Defensins not only have the ability to strengthen the innate immune system but can also enhance the adaptive immune system by chemotaxis of monocytes, T-lymphocytes, dendritic cells and mast cells to the infection site. Defensins will also improve the capacity of macrophage phagocytosis.
 Avian β-defensins
β-defensins are classified in three classes and Avian β-defensins constitute for one of the classes. This division is based on Zhang’s classification and both the length, the homology of the peptides and the gene structure are factors affecting the classification.
Avian β-defensins are separated in avian heterophils and non-heterophils. Avian heterophils can be divided into two sub-classes, depending on the number of present homologous residues in the genome.
Ostriches have a genome containing the gene coding for the antimicrobial peptide, Ostricacin-1. The presence of this peptide indicate that the genes coding for β-defensins have existed for a long time. Ostrich and other ratite species are related to Palaeognathiformes, which is the oldest order of birds living today.
β-defensins genes are found in the genome of both ostrich and mammalians. The genes coding for β-defensins could originate from genes which existed prior diversification of the avian and the mammalian line, which occurred for around 150 million years ago.
The fact that alpha and theta defensins are absence in older vertebrates, like birds and fishes, indicates that defensins must have evolved from the same ancestral gene coding for β-defensins.
Hoover et al (2001) showed that the origin of defensins were molecules similar to β-defensins which are found today, by comparing the amino acids and structures of the origin of β-defensins with β-defensins from insects and α-defensins found in mammalians. The β-defensins found in insects were actually more similar to the origin of defensins compared to α-defensins found in mammalians. The insects lines have been around for a longer time compared to mammalian lines, which suggest that the ancestor of the genes coding for defensins have existed for a long time.
 Human proteins containing this domain
- Selsted ME, White SH, Wimley WC (1995). "Structure, function, and membrane integration of defensins". Curr. Opin. Struct. Biol. 5 (4): 521–527. doi:10.1016/0959-440X(95)80038-7. PMID 8528769.
- Bensch KW, Raida M, Mägert HJ, Schulz-Knappe P, Forssmann WG (July 1995). "hBD-1: a novel beta-defensin from human plasma". FEBS Lett. 368 (2): 331–5. doi:10.1016/0014-5793(95)00687-5. PMID 7628632. http://linkinghub.elsevier.com/retrieve/pii/0014-5793(95)00687-5.
- Hellgren, O., Sheldon, B.C. Locus-specific protocol for nine different innate immune genes (antimicrobial peptides: B-defensins) across passerine bird species reveals within-species coding variation and a case of trans-species polymorphisms. Blackwell Publishing. 11, 686-692
- Ganz, T., 2003.Defensins: antimicrobial peptides of innate immunity. Nature publishing group. 3, 710-720.
- Van Dijk, A., Veldhuizen, E.J.A., Haagsman, H.P. Avian defensins. Elsevier B.V. 124, 1-18
- . Mogensen, H.T. Pathogen recognition and inflammatory signals in innate immune defenses. American society for Microbiology. 22, 240-273
- White, S.H., Wimley, W.C., Selsted, M.E.Structure, function and membrane integration of defensins. Current Biology. 5, 521-527
- Sugiarto, H. Yu, P-L. Avian antimicrobial peptides: the defense role of B-defensins. Elsevier Inc. 323, 721-727
- Yu, P.L., Choudhury, P.L., Ahren, K. Purification and characterization of the antimicrobial peptide, ostricacin, Biotechnol. Lett. 23, 207-210
- Hedges, S.B., Parker, P.H., Sibley, C.G., Kumah, S. Contimental breakup and the ordinal diversification of birds and mammals. Nature. 381, 226-229
- C. A. M., M. Rolfe., J. R. Dorin. Duplication and selection in the evolution of primate β-defensin genes. Genome Biol. 4:R31
- Hoover, D.M., Chertov, O., Lubkowski, J. The structure of human β-defensin-1. Biol. Chem. 279, 39021-39026
- Harder J, Siebert R, Zhang Y, Matthiesen P, Christophers E, Schlegelberger B, Schröder JM (December 1997). "Mapping of the gene encoding human beta-defensin-2 (DEFB2) to chromosome region 8p22-p23.1". Genomics 46 (3): 472–5. doi:10.1006/geno.1997.5074. PMID 9441752. http://linkinghub.elsevier.com/retrieve/pii/S0888-7543(97)95074-6.
 Further reading
- Liu L, Zhao C, Heng HH, Ganz T (August 1997). "The human beta-defensin-1 and alpha-defensins are encoded by adjacent genes: two peptide families with differing disulfide topology share a common ancestry". Genomics 43 (3): 316–20. doi:10.1006/geno.1997.4801. PMID 9268634. http://linkinghub.elsevier.com/retrieve/pii/S0888-7543(97)94801-1.
Beta defensin Provide feedback
The beta defensins are antimicrobial peptides implicated in the resistance of epithelial surfaces to microbial colonisation .
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