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255  structures 7069  species 2  interactions 12610  sequences 19  architectures

Family: Gln-synt_C (PF00120)

Summary: Glutamine synthetase, catalytic domain

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This is the Wikipedia entry entitled "Glutamine synthetase". More...

Glutamine synthetase Edit Wikipedia article

glutamate—ammonia ligase
MN MN ADP PPQ.png
Active site between two monomers of glutamine synthetase from Salmonella typhimurium. Cation binding sites are yellow and orange; ADP is pink; phosphinothricin is blue.[1]
Identifiers
EC number 6.3.1.2
CAS number 9023-70-5
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum
Gene Ontology AmiGO / EGO
Glutamine synthetase,
beta-Grasp domain
Identifiers
Symbol Gln-synt_N
Pfam PF03951
InterPro IPR008147
PROSITE PDOC00162
SCOP 2gls
SUPERFAMILY 2gls
Glutamine synthetase,
catalytic domain
PDB 2gls EBI.jpg
12-subunit enzyme glutamine synthetase from Salmonella typhimurium.[2]
Identifiers
Symbol Gln-synt_C
Pfam PF00120
Pfam clan CL0286
InterPro IPR008146
PROSITE PDOC00162
SCOP 2gls
SUPERFAMILY 2gls
glutamate-ammonia ligase (glutamine synthetase)
Identifiers
Symbol GLUL
Alt. symbols GLNS
Entrez 2752
HUGO 4341
OMIM 138290
PDB 2qc8 (RCSB PDB PDBe PDBj)
RefSeq NM_002065
UniProt P15104
Other data
Locus Chr. 1 q31

Glutamine synthetase (GS) (EC 6.3.1.2)[3] is an enzyme that plays an essential role in the metabolism of nitrogen by catalyzing the condensation of glutamate and ammonia to form glutamine:

Glutamate + ATP + NH3 → Glutamine + ADP + phosphate

Glutamine Synthetase reaction.

Glutamine Synthetase uses ammonia produced by nitrate reduction, amino acid degradation, and photorespiration.[4] The amide group of glutamate is a nitrogen source for the synthesis of glutamine pathway metabolites.[5]

Other reactions may take place via GS. Competition between ammonium ion and water, their binding affinities, and the concentration of ammonium ion, influences glutamine synthesis and glutamine hydrolysis. Glutamine is formed if an ammonium ion attacks the acyl-phosphate intermediate, while glutamate is remade if water attacks the intermediate.[6][7] Ammonium ion binds more strongly than water to GS due to electrostatic forces between a cation and a negatively charged pocket.[4] Another possible reaction is upon NH2OH binding to GS, rather than NH4+, yields γ-glutamylhydroxamate.[6][7]

Structure[edit]

GS Dodecamer
Glutamine Synthetase – 12 subunits.[1]

Glutamine Synthetase can be composed of 8, 10, or 12 identical subunits separated into two face-to-face rings.[6][8][9][10] Bacterial GS are dodecamers with 12 active sites between each monomer.[6] Each active site creates a ‘bifunnel’ which is the site of three distinct substrate binding sites: nucleotide, ammonium ion, and amino acid.[4][6][10][11] ATP binds to the top of the bifunnel that opens to the external surface of GS.[4] Glutamate binds at the bottom of the active site.[7] The middle of the bifunnel contains two sites in which divalent cations bind (Mn+2 or Mg+2). One cation binding site is involved in phosphoryl transfer of ATP to glutamate, while the second stabilizes active GS and helps with the binding of glutamate.[6]

Hydrogen bonding and hydrophobic interactions hold the two rings of GS together. Each subunit possesses a C-terminus and an N-terminus in its sequence. The C-terminus (helical thong) stabilizes the GS structure by inserting into the hydrophobic region of the subunit across in the other ring. The N-terminus is exposed to the solvent. In addition, the central channel is formed via six four-stranded β-sheets composed of anti-parallel loops from the twelve subunits.[6]

Mechanism[edit]

GS catalyzes the ATP-dependent condensation of glutamate with ammonia to yield glutamine.[4] The hydrolysis of ATP drives[8] the first step of a two-part, concerted mechanism.[4][6] ATP phosphorylates glutamate to form ADP and an acyl-phosphate intermediate, γ-glutamyl phosphate, which reacts with ammonia, forming glutamine and inorganic phosphate. ADP and Pi do not dissociate until ammonia binds and glutamine is released.[6]

ATP binds first to the top of the active site near a cation binding site, while glutamate binds near the second cation binding site at the bottom of the active site.[5][7] The presence of ADP causes a conformational shift in GS that stabilizes γ-glutamyl phosphate atom. Ammonium binds strongly to GS only if the acyl-phosphate intermediate is present. Ammonium, rather than ammonia, binds to GS because the binding site is polar and exposed to solvent.[7] In the second step, deprotonation of ammonium allows ammonia to attack the intermediate from its nearby site to form glutamine.[12] Phosphate leaves through the top of the active site, while glutamine leave through the bottom (between two rings).Goodsell, DS (June 2002). "Glutamine Synthetase". RCSB Protein Data Bank. Retrieved 8 May 2010. [7]

Biological Function[edit]

GS is present predominantly in the brain, kidneys, and liver.[4][10] GS in the brain participates in the metabolic regulation of glutamate, the detoxification of brain ammonia, the assimilation of ammonia, recyclization of neurotransmitters, and termination of neurotransmitter signals.[4][13] GS, in the brain, is found primarily in astrocytes.[14] Astrocytes protect neurons against excitotoxicity by taking up excess ammonia and glutamate.[13] In hyperammonemic environments (high levels of ammonia), astroglial swelling occurs.[13][15][16] Different perspectives have approached the problem of astroglial swelling. One study shows that morphological changes occur that increase GS expression in glutamatergic areas or other adaptations that alleviates high levels of glutamate and ammonia.[13] Another perspective is that astrocyte swelling is due to glutamine accumulation. To prevent increased levels of cortical glutamate and cortical water content, a study has been conducted to prevent GS activity in rats by the use of MSO.[15]

Classes[edit]

There seem to be three different classes of GS:[17][18][19]

  • Class I enzymes (GSI) are specific to prokaryotes, and are oligomers of 12 identical subunits.[20] The activity of GSI-type enzyme is controlled by the adenylation of a tyrosine residue. The adenylated enzyme is inactive.[21]

Plants have two or more isozymes of GSII, one of the isozymes is translocated into the chloroplast.

  • Class III enzymes (GSIII) has, currently, only been found in Bacteroides fragilis and in Butyrivibrio fibrisolvens. It is a double-ringed dodecamer of identical chains.[22] It is much larger (about 700 amino acids) than the GSI (450 to 470 amino acids) or GSII (350 to 420 amino acids) enzymes.

While the three classes of GS's are clearly structurally related, the sequence similarities are not so extensive.

Regulation & Inhibition[edit]

Reversible Covalent Modification. A tyrosine residue in each subunit in GS can be modified by adenylylation.[8] Adenylyl transferase catalyzes the adenylylation and phosphorolysis reactions.[8] Adenyl transferase activity is influenced by two regulatory proteins: PA and PD. PA reduces GS activity by attaching an AMP unit to GS. Adenylyl transferase and PD removes the AMP unit. PA and PD may be interconverted via uridylyl transferase.[8] Adenylylated GS is less active than unadenylated GS.[8][11] In the majority of gram-negative bacteria, GS can be modified by adenylylation (some cyanobacteria and green algae or exceptions).[23]

Glutamine Synthetase Dodecamer.
Glutamine Synthetase activity influenced by regulatory proteins.

Inhibition of GS has largely focused on amino site ligands.[6] Other inhibitors are the result of glutamine metabolism: tryptophan, histidine, carbamoyl phosphate, glucosamine-6-phosphate, cytidine triphosphate (CTD), and adenosine monophosphate (AMP).[5][8][24] Other inhibitors/regulators are glycine and alanine. Alanine, glycine, and serine bind to the glutamate substrate site. GDP, AMP, ADP bind to the ATP site.[6] L-serine, L-alanine, and glycine bind to the site for L-glutamate in unadenylated GS. The four amino acids bind to the site by their common atoms, “the main chain” of amino acids.[5] Glutamate is another product of glutamine metabolism; however, glutamate is a substrate for GS inhibiting it to act as a regulator to GS.2 Each inhibitor can reduce the activity of the enzyme; once all final glutamine metabolites are bound to GS, the activity of GS is almost completely inhibited.[8] Many inhibitory input signals allows for fine tuning of GS by reflecting nitrogen levels in the organism.

Feedback regulation distinguishes the difference between two eukaryotic types of GS: brain and non-brain tissues. Non-brain GS responds to end-product feedback inhibition, while brain GS does not.[6] High concentrations of glutamine-dependent metabolites should inhibit GS activity, while low concentrations should activate GS activity.[6]

MSO.
Methionine Sulfoximine acting as an inhibitor to the glutamate binding site.

Inhibitors:

  • Methionine Sulfoximine (MSO): MSO is an inhibitor that binds to the glutamate site. Bound to GS, MSO is phosphorylated by ATP that results in an irreversible, non-covalent inhibition of GS. The S-isomer configuration is more inhibitory.[6] Glutamate entry is blocked into the active site by a stabilization of the flexible loop in the active site by MSO.[7]
  • Phosphinothricin[1](PPT, Glufosinate): Phosphinothricin is an inhibitor that binds to the glutamate site. Glufosinate is used as an herbicide. Glufosinate treated plants die due to a buildup of ammonia and a cessation of photosynthesis.[10]
  • Many synthetic inhibitors are available today.[6]

References[edit]

  1. ^ a b c PDB 1FPY; Gill HS, Eisenberg D (February 2001). "The crystal structure of phosphinothricin in the active site of glutamine synthetase illuminates the mechanism of enzymatic inhibition". Biochemistry 40 (7): 1903–12. doi:10.1021/bi002438h. PMID 11329256. 
  2. ^ PDB 2GLS; Yamashita MM, Almassy RJ, Janson CA, Cascio D, Eisenberg D (October 1989). "Refined atomic model of glutamine synthetase at 3.5 A resolution". J. Biol. Chem. 264 (30): 17681–90. PMID 2572586. 
  3. ^ Eisenberg D, Almassy RJ, Janson CA, Chapman MS, Suh SW, Cascio D, Smith WW (1987). "Some evolutionary relationships of the primary biological catalysts glutamine synthetase and RuBisCO". Cold Spring Harb. Symp. Quant. Biol. 52: 483–90. PMID 2900091. 
  4. ^ a b c d e f g h Liaw SH, Kuo I, Eisenberg D (Nov 1995). "Discovery of the ammonium substrate site on glutamine synthetase, a third cation binding site". Protein Sci. 4 (11): 2358–65. doi:10.1002/pro.5560041114. PMC 2143006. PMID 8563633. 
  5. ^ a b c d Liaw SH, Pan C, Eisenberg D (Jun 1993). "Feedback inhibition of fully unadenylylated glutamine synthetase from Salmonella typhimurium by glycine, alanine, and serine". Proc. Natl. Acad. Sci. USA 90 (11): 4996–5000. doi:10.1073/pnas.90.11.4996. PMC 46640. PMID 8099447. 
  6. ^ a b c d e f g h i j k l m n o Eisenberg D, Gill HS, Pfluegl GM, Rotstein SH (Mar 2000). "Structure-function relationships of glutamine synthetases". Biochim Biophys Acta. 1477 (1-2): 122–45. doi:10.1016/S0167-4838(99)00270-8. PMID 10708854. 
  7. ^ a b c d e f g Liaw SH, Eisenberg D (Jan 1994). "Structural model for the reaction mechanism of glutamine synthetase, based on five crystal structures of enzyme-substrate complexes". Biochemistry 33 (3): 675–81. doi:10.1021/bi00169a007. PMID 7904828. 
  8. ^ a b c d e f g h Stryer L, Berg JM, Tymoczko JL (2007). Biochemistry (6th ed.). San Francisco: W.H. Freeman. pp. 679–706. ISBN 0-7167-8724-5. 
  9. ^ Goodsell DS (June 2002). "Glutamine Synthetase". Molecule of the month. RCSB Protein Data Bank. Retrieved 2010-05-08. 
  10. ^ a b c d e Krajewski WW, Collins R, Holmberg-Schiavone L, Jones TA, Karlberg T, Mowbray SL (Jan 2008). "Crystal structures of mammalian glutamine synthetases illustrate substrate-induced conformational changes and provide opportunities for drug and herbicide design". J Mol Biol. 375 (1): 317–28. doi:10.1016/j.jmb.2007.10.029. PMID 18005987. 
  11. ^ a b Ginsburg A, Yeh J, Hennig SB, Denton MD (Feb 1970). "Some effects of adenylylation on the biosynthetic properties of the glutamine synthetase from Escherichia coli". Biochemistry 9 (3): 633–49. doi:10.1021/bi00805a025. PMID 4906326. 
  12. ^ Hunt JB, Smyrniotis PZ, Ginsburg A, Stadtman ER (Jan 1975). "Metal ion requirement by glutamine synthetase of Escherichia coli in catalysis of gamma-glutamyl transfer". Arch Biochem Biophys. 166 (1): 102–24. doi:10.1016/0003-9861(75)90370-7. PMID 235885. 
  13. ^ a b c d Suarez I, Bodega G, Fernandez B (Aug–Sep 2002). "Glutamine synthetase in brain: effect of ammonia". Neurochem Int. 41 (2-3): 123–42. doi:10.1016/S0197-0186(02)00033-5. PMID 12020613. 
  14. ^ Venkatesh K, Srikanth L, Vengamma B, Chandrasekhar C, Sanjeevkumar A, Mouleshwara Prasad BC, Sarma PV. In vitro differentiation of cultured human CD34+ cells into astrocytes. Neurol India 2013;61:383-8
  15. ^ a b Willard-Mack CL, Koehler RC, Hirata T, et al. (March 1996). "Inhibition of glutamine synthetase reduces ammonia-induced astrocyte swelling in rat". Neuroscience 71 (2): 589–99. doi:10.1016/0306-4522(95)00462-9. PMID 9053810. 
  16. ^ Tanigami H, Rebel A, Martin LJ, Chen TY, Brusilow SW, Traystman RJ, Koehler RC (2005). "Effect of glutamine synthetase inhibition on astrocyte swelling and altered astroglial protein expression during hyperammonemia in rats". Neuroscience 131 (2): 437–49. doi:10.1016/j.neuroscience.2004.10.045. PMC 1819407. PMID 15708485. 
  17. ^ Kumada Y, Benson DR, Hillemann D, Hosted TJ, Rochefort DA, Thompson CJ, Wohlleben W, Tateno Y (April 1993). "Evolution of the glutamine synthetase gene, one of the oldest existing and functioning genes". Proc. Natl. Acad. Sci. U.S.A. 90 (7): 3009–13. doi:10.1073/pnas.90.7.3009. PMC 46226. PMID 8096645. 
  18. ^ Shatters RG, Kahn ML (November 1989). "Glutamine synthetase II in Rhizobium: reexamination of the proposed horizontal transfer of DNA from eukaryotes to prokaryotes". J. Mol. Evol. 29 (5): 422–8. doi:10.1007/BF02602912. PMID 2575672. 
  19. ^ Brown JR, Masuchi Y, Robb FT, Doolittle WF (June 1994). "Evolutionary relationships of bacterial and archaeal glutamine synthetase genes". J. Mol. Evol. 38 (6): 566–76. doi:10.1007/BF00175876. PMID 7916055. 
  20. ^ "GSI structure". Retrieved 2009-03-31. 
  21. ^ InterPro:IPR001637 Glutamine synthetase class-I, adenylation site
  22. ^ van Rooyen JM, Abratt VR, Sewell BT (August 2006). "Three-dimensional structure of a type III glutamine synthetase by single-particle reconstruction". J. Mol. Biol. 361 (4): 796–810. doi:10.1016/j.jmb.2006.06.026. PMID 16879836. 
  23. ^ Ivanovsky RN, Khatipov EA (1994). "Evidence of covalent modification of glutamine synthetase in the purple sulfur bacterium". FEMS Microbiology Letters 122 (1–2): 115–119. doi:10.1111/j.1574-6968.1994.tb07153.x. 
  24. ^ Krishnan IS, Singhal RK, Dua RD (Apr 1986). "Purification and characterization of glutamine synthetase from Clostridium pasteurianum". Biochemistry 35 (7): 1589–99. doi:10.1021/bi00355a021. PMID 2871863. 

External links[edit]

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External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR008146

Glutamine synthetase (EC) (GS) [PUBMED:2900091] plays an essential role in the metabolism of nitrogen by catalyzing the condensation of glutamate and ammonia to form glutamine.

There seem to be three different classes of GS [PUBMED:8096645, PUBMED:2575672, PUBMED:7916055]:

  • Class I enzymes (GSI) are specific to prokaryotes, and are oligomers of 12 identical subunits. The activity of GSI-type enzyme is controlled by the adenylation of a tyrosine residue. The adenylated enzyme is inactive (see INTERPRO).
  • Class II enzymes (GSII) are found in eukaryotes and in bacteria belonging to the Rhizobiaceae, Frankiaceae, and Streptomycetaceae families (these bacteria have also a class-I GS). GSII are octamer of identical subunits. Plants have two or more isozymes of GSII, one of the isozymes is translocated into the chloroplast.
  • Class III enzymes (GSIII) have been found in Bacteroides fragilis. in Butyrivibrio fibrisolvens. It is a hexamer of identical chains and in some protozoa. It is much larger (about 700 amino acids) than the GSI (450 to 470 amino acids) or GSII (350 to 420 amino acids) enzymes.

While the three classes of GS's are clearly structurally related, the sequence similarities are not so extensive.

Gene Ontology

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Domain organisation

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Pfam Clan

This family is a member of clan GCS (CL0286), which has the following description:

This clan represents a superfamily of carboxylate-amine/ammonia ligases [1] that includes Gamma-Glutamylcysteine synthetase (gamma-GCS) and glutamine synthetase (GS). Gamma-Glutamylcysteine synthetase (gamma-GCS) catalyses the first step in the de novo biosynthesis of glutathione.

The clan contains the following 9 members:

Amidoligase_2 ATP-gua_Ptrans COOH-NH2_lig DUF2126 GCS GCS2 Gln-synt_C Glu_cys_ligase Pup_ligase

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(9179)
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(856)
RP35
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RP55
(2250)
RP75
(2691)
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  Seed
(95)
Full
(12610)
Representative proteomes NCBI
(9179)
Meta
(7704)
RP15
(856)
RP35
(1657)
RP55
(2250)
RP75
(2691)
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Seed source: Prosite
Previous IDs: gln-synt;
Type: Domain
Author: Sonnhammer ELL, Griffiths-Jones SR
Number in seed: 95
Number in full: 12610
Average length of the domain: 221.90 aa
Average identity of full alignment: 30 %
Average coverage of the sequence by the domain: 57.41 %

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HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 22.6 22.6
Trusted cut-off 22.6 22.7
Noise cut-off 22.3 22.5
Model length: 259
Family (HMM) version: 19
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Interactions

There are 2 interactions for this family. More...

Gln-synt_C Gln-synt_N

Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Gln-synt_C domain has been found. There are 255 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.

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