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Phosphofructokinase Edit Wikipedia article
- "PFK" redirects here. PFK (Poulet Frit Kentucky) is also the name for KFC in Quebec. For the Polish hip-hop group, see Paktofonika.
The enzyme-catalysed transfer of a phosphoryl group from ATP is an important reaction in a wide variety of biological processes. One enzyme that utilises this reaction is phosphofructokinase (PFK), which catalyses the phosphorylation of fructose-6-phosphate to fructose-1,6- bisphosphate, a key regulatory step in the glycolytic pathway. PFK exists as a homotetramer in bacteria and mammals (where each monomer possesses 2 similar domains) and as an octomer in yeast (where there are 4 alpha- (PFK1) and 4 beta-chains (PFK2), the latter, like the mammalian monomers, possessing 2 similar domains). This protein may use the morpheein model of allosteric regulation.
PFK is about 300 amino acids in length, and structural studies of the bacterial enzyme have shown it comprises two similar (alpha/beta) lobes: one involved in ATP binding and the other housing both the substrate-binding site and the allosteric site (a regulatory binding site distinct from the active site, but that affects enzyme activity). The identical tetramer subunits adopt 2 different conformations: in a 'closed' state, the bound magnesium ion bridges the phosphoryl groups of the enzyme products (ADP and fructose-1,6- bisphosphate); and in an 'open' state, the magnesium ion binds only the ADP, as the 2 products are now further apart. These conformations are thought to be successive stages of a reaction pathway that requires subunit closure to bring the 2 molecules sufficiently close to react.
Deficiency in PFK leads to glycogenosis type VII (Tarui's disease), an autosomal recessive disorder characterised by severe nausea, vomiting, muscle cramps and myoglobinuria in response to bursts of intense or vigorous exercise. Sufferers are usually able to lead a reasonably ordinary life by learning to adjust activity levels.
There are two types of the enzyme:
|Type||Synonyms||EC number||Substrate||Product||Subunit genes|
|PFKL, PFKM, PFKP|
|Phosphofructokinase 2||6-phosphofructo-2-kinase||EC 220.127.116.11||
|PFKFB1, PFKFB2, PFKFB3, PFKFB4|
 See also
- Evans PR, Hellinga HW (1987). "Mutations in the active site of Escherichia coli phosphofructokinase". Nature 327 (6121): 437–439. doi:10.1038/327437a0. PMID 2953977.
- Wegener G, Krause U (2002). "Different modes of activating phosphofructokinase, a key regulatory enzyme of glycolysis, in working vertebrate muscle". Biochem. Soc. Trans. 30 (2): 264–270. PMID 12023862.
- Raben N, Exelbert R, Spiegel R, Sherman JB, Nakajima H, Plotz P, Heinisch J (1995). "Functional expression of human mutant phosphofructokinase in yeast: genetic defects in French Canadian and Swiss patients with phosphofructokinase deficiency". Am. J. Hum. Genet. 56 (1): 131–141. PMC 1801305. PMID 7825568. //www.ncbi.nlm.nih.gov/pmc/articles/PMC1801305/.
- T. Selwood and E. K. Jaffe. (2011). "Dynamic dissociating homo-oligomers and the control of protein function.". Arch. Biochem. Biophys. 519 (2): 131–43. doi:10.1016/j.abb.2011.11.020. PMC 3298769. PMID 22182754. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=22182754.
- Shirakihara Y, Evans PR (1988). "Crystal structure of the complex of phosphofructokinase from Escherichia coli with its reaction products". J. Mol. Biol. 204 (4): 973–994. doi:10.1016/0022-2836(88)90056-3. PMID 2975709.
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This is the Wikipedia entry entitled "Phosphofructokinase 1". More...
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Phosphofructokinase 1 Edit Wikipedia article
|PDB structures||RCSB PDB PDBe PDBsum|
|Gene Ontology||AmiGO / EGO|
Phosphofructokinase-1 (PFK-1) is one of the most important regulatory enzymes (EC 18.104.22.168) of glycolysis. It is an allosteric enzyme made of 4 subunits and controlled by many activators and inhibitors. PFK-1 catalyzes the important "committed" step of glycolysis, the conversion of fructose 6-phosphate and ATP to fructose 1,6-bisphosphate and ADP. Glycolysis is the foundation for respiration, both anaerobic and aerobic. Because phosphofructokinase (PFK) catalyzes the ATP-dependent phosphorylation to convert fructose-6-phosphate into fructose 1,6-bisphosphate and ADP, it is one of the key regulatory and rate limiting step of glycolysis. PFK is able to regulate glycolysis through allosteric inhibition, and in this way, the cell can increase or decrease the rate of glycolysis in response to the cell’s energy requirements. For example high ratio of ATP to ADP will inhibit PFK and glycolysis. There is a key difference between the regulation of PFK in eukaryotes and prokaryotes is that in eukaryotes, PFK is activated by fructose-2,6-bisphosphate. The purpose of fructose-2,6-bisphospate is to supersede ATP inhibition, thus allowing eukaryotes to have greater sensitivity to regulation by hormones like glucagon and insulin.
|β-D-fructose 6-phosphate||Phosphofructokinase-1||β-D-fructose 1,6-bisphosphate|
Mammalian PFK1 is a 340kd tetramer composed of three types of subunit: muscle (M), liver (L), and platelet (P). The composition of the PFK1 tetramer differs according to the tissue type it is present in. For example, mature muscle expresses only the M isozyme, therefore, the muscle PFK1 is composed solely of homotetramers of M4. The liver and kidneys express predominantly the L isoform. Erythrocytes express both M and L subunits which randomly tetramerize to form M4, L4 and the three hybrid forms of the enzyme (ML3, M2L2, M3L). As a result, the kinetic and regulatory properties of the various isoenzymes pools are dependent on subunit composition. Tissue-specific changes in PFK activity and isoenzymic content contribute significantly to the diversities of glycolytic and gluconeogenic rates which have been observed for different tissues.
PFK1 is an allosteric enzyme and has a structure similar to that of hemoglobin insofar as it is a dimer of a dimer. One half of each dimer contains the ATP binding site whereas the other half the substrate (fructose-6-phosphate or (F6P)) binding site as well as a separate allosteric binding site.
Each subunit of the tetramer is 319 amino acids and consists of two domain, one that binds the substrate ATP, and the other that binds fructose-6-phosphate. Each domain is a b barrel, and has cylindrical b sheet surrounded by alpha helices.
On the opposite side of the each subunit from each active site is the allosteric site, at the interface between subunits in the dimer. This is where ADP binds. The N-terminal domain has a catalytic role binding the ATP, and the C-terminal has a regulatory role 
PFK1 is an allosteric enzyme whose activity can be described using the symmetry model of allosterism whereby there is a concerted transition from an enzymatically inactive T-state to the active R-state. F6P binds with a high affinity to the R state but not the T state enzyme. For every molecule of F6P that binds to PFK1, the enzyme progressively shifts from T state to the R state. Thus a graph plotting PFK1 activity against increasing F6P concentrations would adopt the sigmoidal curve shape traditionally associated with allosteric enzymes.
PFK1 belongs to the family of phosphotransferases and it catalyzes the transfer of γ-phosphate from ATP to fructose-6-phosphate. The PFK1 active site comprises both the ATP-Mg2+ and the F6P binding sites. Some proposed residues involved with substrate binding in E. coli FPK1 include Asp127 and Arg171. In B. stearothermophilus PFK1, the positively charged side chain of Arg162 residue forms a hydrogen-bonded salt bridge with the negatively charged phosphate group of F6P, an interaction which stabilizes the R state relative to the T state and is partly responsible for the homotropic effect of F6P binding. In the T state, enzyme conformation shifts slightly such that the space previously taken up by the Arg162 is replaced with Glu161. This swap in positions between adjacent amino acid residues inhibits the ability of F6P to bind the enzyme.
Allosteric activators such as AMP and ADP bind to the allosteric site as to facilitate the formation of the R state by inducing structural changes in the enzyme. Similarly, inhibitors such as ATP and PEP bind to the same allosteric site and facilitate the formation of the T state, thereby inhibiting enzyme activity.
PFK1 is the most important control site in the mammalian glycolytic pathway. This step is subject to extensive regulation since it is not only highly endergonic under physiological conditions, but also because it is a committed step - the first irreversible reaction unique to the glycolytic pathway. This leads to a precise control of glucose and the other monosaccharides galactose and fructose going down the glycolytic pathway. Before this enzyme's reaction, glucose-6-phosphate can potentially travel down the pentose phosphate pathway, or be converted to glucose-1-phosphate for glycogenesis.
PFK1 is allosterically inhibited by high levels of ATP but AMP reverses the inhibitory action of ATP. Therefore, the activity of the enzyme increases when the cellular ATP/AMP ratio is lowered. Glycolysis is thus stimulated when energy charge falls. PFK1 has two sites with different affinities for ATP which is both a substrate and an inhibitor.
PFK1 is also inhibited by low pH levels which augment the inhibitory effect of ATP. The pH falls when muscle is functioning anaerobically and producing excessive quantities of lactic acid. This inhibitory effect serves to protect the muscle from damage that would result from the accumulation of too much acid.
Finally, PFK1 is allosterically inhibited by both PEP and citrate. Phosphoenolpyruvic acid is a product further downstream the glycolytic pathway and citrate is an early intermediate in the citric acid cycle. Citrate buildup is a sign of the citric acid cycle reaching saturation and thus glycolysis slows down as there is no longer any need to commit more glucose to degradation.
PFK1 is allosterically activated by a high concentration of AMP, but the most potent activator is fructose 2,6-bisphosphate, which is also produced from fructose-6-phosphate by PFK2. Hence, an abundance of F6P results in a higher concentration of fructose 2,6-bisphosphate (F-2,6-BP). The binding of F-2,6-BP increases the affinity of PFK1 for F6P and diminishes the inhibitory effect of ATP. This is an example of feedforward stimulation as glycolysis is accelerated when glucose is abundant.
PFK is inhibited by glucagon through repression of synthesis. Glucagon activates protein kinase A which, in turn, shuts off the kinase activity of PFK2. This reverses any synthesis of F-2,6-BP from F6P and thus inhibits PFK1 activity.
The precise regulation of PFK1 prevents glycolysis and gluconeogenesis from occurring simultaneously. However, there is substrate cycling between F6P and F-1,6-BP. Fructose-1,6-bisphosphatase (FBPase) catalyzes the hydrolysis of F-1,6-BP back to F6P, the reverse reaction catalyzed by PFK1. There is a small amount of FBPase activity during glycolysis and some PFK1 activity during gluconeogenesis. This cycle allows for the amplification of metabolic signals as well as the generation of heat by ATP hydrolysis.
Serotonin (5-HT) increases PFK by binding to the 5-HT(2A) receptor, causing the tyrosine residue of PFK to be phosphorylated via phospholipase C. This in turn redistributes PFK within the skeletal muscle cells. Because PFK regulates glycolytic flux, serotonin plays a regulatory role in glycolysis 
There are three phosphofructokinase genes in humans:
 Clinical signficance
Tarui Disease: Muscles which store glycogen require Tarui disease is a glycogen storage disease with symptoms including muscle weakness (myopathy) and exercise induced cramping and spasms, myoglobinuria (presence of myoglobin in urine, indicating muscle destruction) and compensated hemolysis. ATP is a natural allosteric inhibitor of PFK, in order to prevent unnecessary production of ATP through glycolysis. However, a mutation in Asp(543)Ala can result in ATP having a stronger inhibitory effect (due to increased binding to PFK’s inhibitory allosteric binding site)
Phosphofructokinase mutation and cancer: In order for cancer cells to meet their energy requirements due to their rapid cell growth and division, they survive more effectively when they have a hyperactive phosphofructokinase 1 enzyme. When cancer cells grow and divide quickly, they initially do not have as much blood supply, and can thus have hypoxia (oxygen deprivation), and this triggers O-GlcNAcylation at serine 529 of PFK, giving a selective growth advantage to cancer cells.
Herpes simplex type 1 and phosphofructokinase: Some viruses, including HIV, HCMV, Mayaro, and HCMV affect cellular metabolic pathways such as glycolysis by a MOI-dependent increase in the activity of PFK. The mechanism that Herpes increases PFK activity is by phosphorylating the enzyme at the serine residues. The HSV-1 induced glycolysis increases ATP content, which is critical for the virus’s replication 
 See also
- PFK2 (converts fructose 6-phosphate to fructose 2,6-bisphosphate through on site, or the opposite, on another site)
- PFP (reversibly interconverts fructose 6-phosphate and fructose 1,6-bisphosphate using inorganic pyrophosphate rather than ATP)
- fructose bisphosphatase (hydrolyses fructose 1,6-bisphosphate to fructose 6-phosphate)
- Usenik A, Legiša M (Nov 2010). "Evolution of allosteric citrate binding sites on 6-phosphofructo-1-kinase". In Kobe, Bostjan. PLoS One. 5 (11): 677–683. doi:10.1371/journal.pone.0015447. PMC 1149058. PMID 21124851.
- Stryer L, Berg JM, Tymoczko JL (2007). Biochemistry (Sixth ed.). San Francisco: W.H. Freeman. ISBN 0-7167-8724-5.
- Dunaway GA, Kasten TP, Sebo T, Trapp R (May 1988). "Analysis of the phosphofructokinase subunits and isoenzymes in human tissues". Biochem. J. 251 (3): 677–83. PMC 1149058. PMID 2970843.
- PDB 4pfk; Evans PR, Farrants GW, Hudson PJ (June 1981). "Phosphofructokinase: structure and control". Philos. Trans. R. Soc. Lond., B, Biol. Sci. 293 (1063): 53–62. doi:10.1098/rstb.1981.0059. PMID 6115424. Lay summary – PDB Molecule of the Month.
- Shirakihara Y, Evans PR (December 1988). "Crystal structure of the complex of phosphofructokinase from Escherichia coli with its reaction products". J. Mol. Biol. 204 (4): 973–94. doi:10.1016/0022-2836(88)90056-3. PMID 2975709.
- Banaszak K, Mechin I, Obmolova G, Oldham M, Chang SH, Ruiz T, Radermacher M, Kopperschläger G, Rypniewski W (March 2011). "The crystal structures of eukaryotic phosphofructokinases from baker's yeast and rabbit skeletal muscle". J Mol Biol. 402 (7): 284–97. doi:10.1016/j.jmb.2011.01.019. PMC 1149058. PMID 21241708.
- Peskov K, Goryanin I, Demin O (August 2008). "Kinetic model of phosphofructokinase-1 from Escherichia coli". J Bioinform Comput Biol 6 (4): 843–67. doi:10.1142/S0219720008003643. PMID 18763746.
- Hellinga HW, Evans PR (1987). "Mutations in the active site of Escherichia coli phosphofructokinase". Nature 327 (6121): 437–9. doi:10.1038/327437a0. PMID 2953977.
- Phong WY, Lin W, Rao SP, Dick T, Alonso S, Pethe K (Aug 2013). "Characterization of Phosphofructokinase Activity in Mycobacterium tuberculosis Reveals That a Functional Glycolytic Carbon Flow Is Necessary to Limit the Accumulation of Toxic Metabolic Intermediates under Hypoxia". In Parish, Tanya. PLos One 8 (2): 1198–206. doi:10.1371/journal.pone.0056037. PMC 1149058. PMID 23409118.
- Papagianni M, Avramidis N (May 2012). "Engineering the central pathways in Lactococcus lactis: functional expression of the phosphofructokinase (pfk) and alternative oxidase (aox1) genes from Aspergillus niger in Lactococcus lactis facilitates improved carbon conversion rates under oxidizing conditions". Enzyme Microb Technol. 51 (113): 125–30. doi:10.1016/j.enzmictec.2012.04.007. PMC 1149058. PMID 22759530.
- Coelho WS, Sola-Penna M (Jan 2013). "Serotonin regulates 6-phosphofructo-1-kinase activity in a PLC-PKC-CaMK II- and Janus kinase-dependent signaling pathway". Mol Cell Biochem. 372 (1–2): 211–20. doi:10.1007/s11010-012-1462-0. PMC 1149058. PMID 23010892.
- Nakajima H, Raben N, Hamaguchi T, Yamasaki T (March 2002). "Phosphofructokinase deficiency; past, present and future". Curr. Mol. Med. 2 (2): 197–212. doi:10.2174/1566524024605734. PMID 11949936.
- Bruser A, KirchbergerJ, Schoneberg T (Oct 2012). "AAltered allosteric regulation of muscle 6-phosphofructokinase causes Tarui disease". Biochem Biophys Res Commun 427 (1): 133–7. doi:10.1016/j.bbrc.2012.09.024. PMC 1149058. PMID 22995305.
- Brüser A, Kirchberger J, Schöneberg T (October 2012). "Altered allosteric regulation of muscle 6-phosphofructokinase causes Tarui disease". Biochem. Biophys. Res. Commun. 427 (1): 133–7. doi:10.1016/j.bbrc.2012.09.024. PMID 22995305.
- Yi W, Clark PM, Mason DE, Keenan MC, Hill C, Goddard WA 3rd, Peters EC, Driggers EM, Hsieh-Wilson LC (Aug 2012). "Phosphofructokinase 1 glycosylation regulates cell growth and metabolism". Science 337 (6097): 975–80. doi:10.1126/science. PMC 1149058. PMID 22923583.
- Gomez LS, Zancan P, Marcondes MC, Ramos-Santos L, Meyer-Fernandes JR, Sola-Penna M, Da Silva D (Feb 2013). "Resveratrol decreases breast cancer cell viability and glucose metabolism by inhibiting 6-phosphofructo-1-kinase". Biochimie. 1822 (8): 1198–206. doi:10.1016/j.biochi.2013.02.013. PMC 1149058. PMID 23454376.
- Vaz CV, Alves MG, Marques R, Moreira PI, Oliveira PF, Maia CJ, Socorro S (Feb 2013). "Resveratrol decreases breast cancer cell viability and glucose metabolism by inhibiting 6-phosphofructo-1-kinase". Int J Biochem Cell Biol. 44 (11): 2077–84. doi:10.1016/j.biocel.2012.08.013. PMC 1149058. PMID 22964025.
- Abrantes JL, Alves CM, Costa J, Almeida FC, Sola-Penna M, Fontes CF, Souza TM (Aug 2012). "Herpes simplex type 1 activates glycolysis through engagement of the enzyme 6-phosphofructo-1-kinase (PFK-1)". Biochim Biophys Acta. 1822 (8): 1198–206. doi:10.1016/j.bbadis.2012.04.011. PMC 1149058. PMID 22542512.
- Phosphofructokinase-1 at the US National Library of Medicine Medical Subject Headings (MeSH)
- Proteopedia.org Phosphofructokinase
Phosphofructokinase Provide feedback
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External database links
This tab holds annotation information from the InterPro database.
InterPro entry IPR000023The enzyme-catalysed transfer of a phosphoryl group from ATP is an important reaction in a wide variety of biological processes [PUBMED:2953977]. One enzyme that utilises this reaction is phosphofructokinase (PFK), which catalyses the phosphorylation of fructose-6-phosphate to fructose-1,6- bisphosphate, a key regulatory step in the glycolytic pathway [PUBMED:12023862, PUBMED:7825568]. PFK exists as a homotetramer in bacteria and mammals (where each monomer possesses 2 similar domains), and as an octomer in yeast (where there are 4 alpha- (PFK1) and 4 beta-chains (PFK2), the latter, like the mammalian monomers, possessing 2 similar domains [PUBMED:7825568]).
PFK is ~300 amino acids in length, and structural studies of the bacterial enzyme have shown it comprises two similar (alpha/beta) lobes: one involved in ATP binding and the other housing both the substrate-binding site and the allosteric site (a regulatory binding site distinct from the active site, but that affects enzyme activity). The identical tetramer subunits adopt 2 different conformations: in a 'closed' state, the bound magnesium ion bridges the phosphoryl groups of the enzyme products (ADP and fructose-1,6- bisphosphate); and in an 'open' state, the magnesium ion binds only the ADP [PUBMED:2975709], as the 2 products are now further apart. These conformations are thought to be successive stages of a reaction pathway that requires subunit closure to bring the 2 molecules sufficiently close to react [PUBMED:2975709].
Deficiency in PFK leads to glycogenosis type VII (Tauri's disease), an autosomal recessive disorder characterised by severe nausea, vomiting, muscle cramps and myoglobinuria in response to bursts of intense or vigorous exercise [PUBMED:7825568]. Sufferers are usually able to lead a reasonably ordinary life by learning to adjust activity levels [PUBMED:7825568].
The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.
|Cellular component||6-phosphofructokinase complex (GO:0005945)|
|Molecular function||6-phosphofructokinase activity (GO:0003872)|
|Biological process||glycolysis (GO:0006096)|
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|Number in seed:||12|
|Number in full:||6795|
|Average length of the domain:||268.50 aa|
|Average identity of full alignment:||35 %|
|Average coverage of the sequence by the domain:||74.02 %|
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build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
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