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24  structures 266  species 2  interactions 1212  sequences 17  architectures

Family: G-gamma (PF00631)

Summary: GGL domain

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This is the Wikipedia entry entitled "GGL domain". More...

GGL domain Edit Wikipedia article

1b9x opm.gif
Phosducin - transducin beta-gamma complex. Gamma subunit (GGL domain) colored red.
Identifiers
Symbol G-gamma
Pfam PF00631
InterPro IPR001770
SMART GGL
PROSITE PDOC01002
SCOP 1gp2
SUPERFAMILY 1gp2
OPM protein 2bcj
CDD cd00068

GGL domain is domain found in the gamma subunit of the heterotrimeric G protein complex and in regulators of G protein signaling RGS proteins.[1]

Human proteins containing this domain[edit]

See also[edit]

References[edit]

  1. ^ Snow BE, Krumins AM, Brothers GM, Lee SF, Wall MA, Chung S, Mangion J, Arya S, Gilman AG, Siderovski DP (1998). "A G protein γ subunit-like domain shared between RGS11 and other RGS proteins specifies binding to Gβ5 subunits". Proc. Natl. Acad. Sci. U.S.A. 95 (22): 13307–12. doi:10.1073/pnas.95.22.13307. PMC 23793. PMID 9789084. 

Further reading[edit]

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

GGL domain Provide feedback

G-protein gamma like domains (GGL) are found in the gamma subunit of the heterotrimeric G protein complex and in regulators of G protein signaling (RGS) proteins [1]. It is also found fused to an inactive Galpha in the Dictyostelium protein gbqA [2]. G-gamma likely shares a common origin with the helical N-terminal unit of G-beta [2]. All organisms that posses a G-beta possess a G-gamma [2].

Literature references

  1. Snow BE, Krumins AM, Brothers GM, Lee SF, Wall MA, Chung S, Mangion J, Arya S, Gilman AG, Siderovski DP; , Proc Natl Acad Sci U S A 1998;95:13307-13312.: A G protein gamma subunit-like domain shared between RGS11 and other RGS proteins specifies binding to Gbeta5 subunits. PUBMED:9789084 EPMC:9789084

  2. Anantharaman V, Abhiman S, de Souza RF, Aravind L;, Gene. 2011;475:63-78.: Comparative genomics uncovers novel structural and functional features of the heterotrimeric GTPase signaling system. PUBMED:21182906 EPMC:21182906


External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR015898

This entry represents the G protein gamma subunit and the GGL (G protein gamma-like) domain, which are related in sequence and are comprised of an extended alpha-helical polypeptide. The G protein gamma subunit forms a stable dimer with the beta subunit, but it does not make any contact with the alpha subunit, which contacts the opposite face of the beta subunit. The GGL domain is found in several RGS (regulators of G protein signaling) proteins. GGL domains can interact with beta subunits to form novel dimers that prevent gamma subunit binding, and may prevent heterotrimer formation by inhibiting alpha subunit binding. The interaction between G protein beta-5 neuro-specific isoforms and RGS GGL domains may represent a general mode of binding between beta-propeller proteins and their partners [PUBMED:11331068].

Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the NCBI sequence database, and our metagenomics sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(50)
Full
(1212)
Representative proteomes NCBI
(1015)
Meta
(0)
RP15
(129)
RP35
(220)
RP55
(399)
RP75
(681)
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1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(50)
Full
(1212)
Representative proteomes NCBI
(1015)
Meta
(0)
RP15
(129)
RP35
(220)
RP55
(399)
RP75
(681)
Alignment:
Format:
Order:
Sequence:
Gaps:
Download/view:

Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(50)
Full
(1212)
Representative proteomes NCBI
(1015)
Meta
(0)
RP15
(129)
RP35
(220)
RP55
(399)
RP75
(681)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download    
Gzipped Download   Download   Download   Download   Download   Download   Download    

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

External links

MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER3.

Pfam alignments:

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Ananthraman V
Previous IDs: G_protein_gamma;
Type: Domain
Author: Bateman A, Ananthraman V
Number in seed: 50
Number in full: 1212
Average length of the domain: 65.80 aa
Average identity of full alignment: 28 %
Average coverage of the sequence by the domain: 29.38 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 22.3 22.3
Trusted cut-off 22.6 22.4
Noise cut-off 21.9 21.7
Model length: 68
Family (HMM) version: 17
Download: download the raw HMM for this family

Species distribution

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Interactions

There are 2 interactions for this family. More...

RGS WD40

Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the G-gamma domain has been found. There are 24 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.

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