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79  structures 511  species 1  interaction 15670  sequences 989  architectures

Family: BTB (PF00651)

Summary: BTB/POZ domain

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This is the Wikipedia entry entitled "BTB/POZ domain". More...

BTB/POZ domain Edit Wikipedia article

BTB/POZ domain
PDB 1buo EBI.jpg
Structure of the BTB domain from PLZF.[1]
Identifiers
Symbol BTB
Pfam PF00651
InterPro IPR013069
PROSITE PS50097
SCOP 1buo
SUPERFAMILY 1buo

The BTB/POZ domain is a common structural domain contained within some proteins.

The BTB (for BR-C, ttk and bab)[2] or POZ (for Pox virus and Zinc finger)[3] domain is present near the N-terminus of a fraction of zinc finger proteins and in proteins that contain the Kelch motif and a family of pox virus proteins. The BTB/POZ domain mediates homomeric dimerisation and in some instances heteromeric dimerisation.[3] The structure of the dimerised PLZF BTB/POZ domain has been solved and consists of a tightly intertwined homodimer. The central scaffolding of the protein is made up of a cluster of alpha-helices flanked by short beta-sheets at both the top and bottom of the molecule.[1] BTB/POZ domains from several zinc finger proteins have been shown to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes including N-CoR and SMRT.[4][5][6] The POZ or BTB domain is also known as BR-C/Ttk or ZiN.

References[edit]

  1. ^ a b Ahmad KF, Engel CK, Privé GG (October 1998). "Crystal structure of the BTB domain from PLZF". Proc. Natl. Acad. Sci. U.S.A. 95 (21): 12123–8. doi:10.1073/pnas.95.21.12123. PMC 22795. PMID 9770450. 
  2. ^ Zollman S, Godt D, Privé GG, Couderc JL, Laski FA (October 1994). "The BTB domain, found primarily in zinc finger proteins, defines an evolutionarily conserved family that includes several developmentally regulated genes in Drosophila". Proc. Natl. Acad. Sci. U.S.A. 91 (22): 10717–21. doi:10.1073/pnas.91.22.10717. PMC 45093. PMID 7938017. 
  3. ^ a b Bardwell VJ, Treisman R (July 1994). "The POZ domain: a conserved protein-protein interaction motif". Genes Dev. 8 (14): 1664–77. doi:10.1101/gad.8.14.1664. PMID 7958847. 
  4. ^ Deweindt C, Albagli O, Bernardin F, Dhordain P, Quief S, Lantoine D, Kerckaert JP, Leprince D (December 1995). "The LAZ3/BCL6 oncogene encodes a sequence-specific transcriptional inhibitor: a novel function for the BTB/POZ domain as an autonomous repressing domain". Cell Growth Differ. 6 (12): 1495–503. PMID 9019154. 
  5. ^ Huynh KD, Bardwell VJ (November 1998). "The BCL-6 POZ domain and other POZ domains interact with the co-repressors N-CoR and SMRT". Oncogene 17 (19): 2473–84. doi:10.1038/sj.onc.1202197. PMID 9824158. 
  6. ^ Wong CW, Privalsky ML (October 1998). "Components of the SMRT corepressor complex exhibit distinctive interactions with the POZ domain oncoproteins PLZF, PLZF-RARalpha, and BCL-6". J. Biol. Chem. 273 (42): 27695–702. doi:10.1074/jbc.273.42.27695. PMID 9765306. 

This article incorporates text from the public domain Pfam and InterPro IPR013069

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

BTB/POZ domain Provide feedback

The BTB (for BR-C, ttk and bab) [1] or POZ (for Pox virus and Zinc finger) [2] domain is present near the N-terminus of a fraction of zinc finger (PF00096) proteins and in proteins that contain the PF01344 motif such as Kelch and a family of pox virus proteins. The BTB/POZ domain mediates homomeric dimerisation and in some instances heteromeric dimerisation [2]. The structure of the dimerised PLZF BTB/POZ domain has been solved and consists of a tightly intertwined homodimer. The central scaffolding of the protein is made up of a cluster of alpha-helices flanked by short beta-sheets at both the top and bottom of the molecule [3]. POZ domains from several zinc finger proteins have been shown to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes including N-CoR and SMRT [4,5,6]. The POZ or BTB domain is also known as BR-C/Ttk or ZiN.

Literature references

  1. Zollman S, Godt D, Prive GG, Couderc JL, Laski FA; , Proc Natl Acad Sci U S A 1994;91:10717-10721.: The BTB domain, found primarily in zinc finger proteins, defines an evolutionarily conserved family that includes several developmentally regulated genes in Drosophila. PUBMED:7938017 EPMC:7938017

  2. Bardwell VJ, Treisman R; , Genes Dev 1994;8:1664-1677.: The POZ domain: a conserved protein-protein interaction motif. PUBMED:7958847 EPMC:7958847

  3. Ahmad KF, Engel CK, Prive GG; , Proc Natl Acad Sci U S A 1998;95:12123-12128.: Crystal structure of the BTB domain from PLZF. PUBMED:9770450 EPMC:9770450

  4. Deweindt C, Albagli O, Bernardin F, Dhordain P, Quief S, Lantoine D, Kerckaert JP, Leprince D; , Cell Growth Differ 1995;6:1495-1503.: The LAZ3/BCL6 oncogene encodes a sequence-specific transcriptional inhibitor: a novel function for the BTB/POZ domain as an autonomous repressing domain. PUBMED:9019154 EPMC:9019154

  5. Huynh KD, Bardwell VJ; , Oncogene 1998;17:2473-2484.: The BCL-6 POZ domain and other POZ domains interact with the co-repressors N-CoR and SMRT. PUBMED:9824158 EPMC:9824158

  6. Wong CW, Privalsky ML; , J Biol Chem 1998;273:27695-27702.: Components of the SMRT corepressor complex exhibit distinctive interactions with the POZ domain oncoproteins PLZF, PLZF-RARalpha, and BCL-6. PUBMED:9765306 EPMC:9765306

  7. Stogios PJ, Downs GS, Jauhal JJ, Nandra SK, Prive GG; , Genome Biol. 2005;6:R82.: Sequence and structural analysis of BTB domain proteins. PUBMED:16207353 EPMC:16207353


External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR013069

The BTB (for BR-C, ttk and bab) [PUBMED:7938017] or POZ (for Pox virus and Zinc finger) [PUBMED:7958847] domain is present near the N terminus of a fraction of zinc finger (INTERPRO) proteins and in proteins that contain the INTERPRO motif such as Kelch and a family of pox virus proteins. The BTB/POZ domain mediates homomeric dimerisation and in some instances heteromeric dimerisation [PUBMED:7958847]. The structure of the dimerised PLZF BTB/POZ domain has been solved and consists of a tightly intertwined homodimer. The central scaffolding of the protein is made up of a cluster of alpha-helices flanked by short beta-sheets at both the top and bottom of the molecule [PUBMED:9770450]. POZ domains from several zinc finger proteins have been shown to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes including N-CoR and SMRT [PUBMED:9019154, PUBMED:9824158, PUBMED:9765306]. The POZ or BTB domain is also known as BR-C/Ttk or ZiN.

Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan POZ (CL0033), which has the following description:

The POZ domain is found in a variety of transcription factors. POZ domains are also found in the tetramerisation domain of voltage gated K+ channels. In general these domains mediate homo-oligomerisation.

The clan contains the following 5 members:

BACK BTB BTB_2 DUF3342 Skp1_POZ

Alignments

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(96)
Full
(15670)
Representative proteomes NCBI
(15120)
Meta
(88)
RP15
(2968)
RP35
(4235)
RP55
(7306)
RP75
(10101)
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Format an alignment

  Seed
(96)
Full
(15670)
Representative proteomes NCBI
(15120)
Meta
(88)
RP15
(2968)
RP35
(4235)
RP55
(7306)
RP75
(10101)
Alignment:
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Order:
Sequence:
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(96)
Full
(15670)
Representative proteomes NCBI
(15120)
Meta
(88)
RP15
(2968)
RP35
(4235)
RP55
(7306)
RP75
(10101)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

External links

MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER3.

Pfam alignments:

HMM logo

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Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Prosite
Previous IDs: none
Type: Domain
Author: Bateman A, Bardwell VJ
Number in seed: 96
Number in full: 15670
Average length of the domain: 105.10 aa
Average identity of full alignment: 20 %
Average coverage of the sequence by the domain: 20.57 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 20.5 20.5
Trusted cut-off 20.5 20.5
Noise cut-off 20.4 20.4
Model length: 111
Family (HMM) version: 26
Download: download the raw HMM for this family

Species distribution

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Interactions

There is 1 interaction for this family. More...

BTB

Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the BTB domain has been found. There are 79 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.

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