Summary: AIR carboxylase
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AIR carboxylase Provide feedback
Members of this family catalyse the decarboxylation of 1-(5-phosphoribosyl)-5-amino-4-imidazole-carboxylate (AIR). This family catalyse the sixth step of de novo purine biosynthesis. Some members of this family contain two copies of this domain.
Mathews II, Kappock TJ, Stubbe J, Ealick SE; , Structure Fold Des 1999;7:1395-1406.: Crystal structure of Escherichia coli PurE, an unusual mutase in the purine biosynthetic pathway. PUBMED:10574791 EPMC:10574791
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This tab holds annotation information from the InterPro database.
InterPro entry IPR000031
Phosphoribosylaminoimidazole carboxylase is a fusion protein in plants and fungi, but consists of two non-interacting proteins in bacteria, PurK and PurE, which were previously thought to be subunits of a single enzyme, AIR carboxylase [PUBMED:9219341].
PurK, N5-carboxyaminoimidazole ribonucleotide (N5_CAIR) synthetase, catalyses the conversion of 5-aminoimidazole ribonucleotide (AIR), ATP, and bicarbonate to N5-CAIR, ADP, and Pi. PurE catalyses the reversible conversion of N5-CAIR and CAIR [PUBMED:10074353], the sixth step of de novo purine biosynthesis. In the presence of high concentrations of bicarbonate, PurE is reported able to convert AIR to CAIR directly and without ATP [PUBMED:7918411].
The crystal structure of PurE indicates a unique quaternary structure that confirms the octameric nature of the enzyme [PUBMED:10574791].
|Molecular function||5-(carboxyamino)imidazole ribonucleotide mutase activity (GO:0034023)|
|Biological process||'de novo' IMP biosynthetic process (GO:0006189)|
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|Seed source:||Pfam-B_462 (release 2.1)|
|Author:||Bateman A, Griffiths-Jones SR|
|Number in seed:||224|
|Number in full:||5292|
|Average length of the domain:||147.40 aa|
|Average identity of full alignment:||46 %|
|Average coverage of the sequence by the domain:||73.54 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||15|
|Download:||download the raw HMM for this family|
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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the AIRC domain has been found. There are 106 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.
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