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0  structures 3474  species 0  interactions 5798  sequences 12  architectures

Family: Bax1-I (PF01027)

Summary: Inhibitor of apoptosis-promoting Bax1

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Inhibitor of apoptosis-promoting Bax1 Provide feedback

Programmed cell-death involves a set of Bcl-2 family proteins, some of which inhibit apoptosis (Bcl-2 and Bcl-XL) and some of which promote it (Bax and Bak). Human Bax inhibitor, BI-1, is an evolutionarily conserved integral membrane protein containing multiple membrane-spanning segments predominantly localised to intracellular membranes. It has 6-7 membrane-spanning domains. The C termini of the mammalian BI-1 proteins are comprised of basic amino acids resembling some nuclear targeting sequences, but otherwise the predicted proteins lack motifs that suggest a function. As plant BI-1 appears to localise predominantly to the ER, we hypothesized that plant BI-1 could also regulate cell death triggered by ER stress [2]. BI-1 appears to exert its effect through an interaction with calmodulin [3]. The budding yeast member of this family has been found unexpectedly to encode a BH3 domain-containing protein (Ybh3p) that regulates the mitochondrial pathway of apoptosis in a phylogenetically conserved manner [4].

Literature references

  1. Kawai-Yamada M, Ohori Y, Uchimiya H;, Plant Cell. 2004;16:21-32.: Dissection of Arabidopsis Bax inhibitor-1 suppressing Bax-, hydrogen peroxide-, and salicylic acid-induced cell death. PUBMED:14671021 EPMC:14671021

  2. Watanabe N, Lam E;, Plant Signal Behav. 2008;3:564-566.: Arabidopsis Bax inhibitor-1: A rheostat for ER stress-induced programmed cell death. PUBMED:19704470 EPMC:19704470

  3. Watanabe N, Lam E;, Int J Mol Sci. 2009;10:3149-3167.: Bax inhibitor-1, a conserved cell death suppressor, is a key molecular switch downstream from a variety of biotic and abiotic stress signals in plants. PUBMED:19742129 EPMC:19742129

  4. Buttner S, Ruli D, Vogtle FN, Galluzzi L, Moitzi B, Eisenberg T, Kepp O, Habernig L, Carmona-Gutierrez D, Rockenfeller P, Laun P, Breitenbach M, Khoury C, Frohlich KU, Rechberger G, Meisinger C, Kroemer G, Madeo F;, EMBO J. 2011;30:2779-2792.: A yeast BH3-only protein mediates the mitochondrial pathway of apoptosis. PUBMED:21673659 EPMC:21673659


External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR006214

Bax inhibitor-1 (BI-1) [PUBMED:8530040] is a suppressor of apoptosis that interacts with BCL2 and BCL-X. Human Bax BI-1 is an evolutionarily conserved integral membrane protein containing multiple membrane-spanning segments predominantly localised to intracellular membranes. It has 6-7 membrane-spanning domains. The C termini of the mammalian BI-1 proteins are comprised of basic amino acids resembling some nuclear targeting sequences, but otherwise the predicted proteins lack motifs that suggest this function. As plant BI-1 appears to localise predominantly to the ER, we hypothesized that plant BI-1 could also regulate cell death triggered by ER stress [PUBMED:19704470]. BI-1 appears to exert its effect through an interaction with calmodulin [PUBMED:19742129].

This entry represents BI-1 and related sequences, including lifeguard proteins, which also act as apoptotic regulators.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan Apoptosis-Inhib (CL0453), which has the following description:

This family includes members which are Bax inhibitors.

The clan contains the following 2 members:

Bax1-I BaxI_1

Alignments

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(511)
Full
(5798)
Representative proteomes NCBI
(4003)
Meta
(2434)
RP15
(437)
RP35
(786)
RP55
(1139)
RP75
(1486)
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Format an alignment

  Seed
(511)
Full
(5798)
Representative proteomes NCBI
(4003)
Meta
(2434)
RP15
(437)
RP35
(786)
RP55
(1139)
RP75
(1486)
Alignment:
Format:
Order:
Sequence:
Gaps:
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(511)
Full
(5798)
Representative proteomes NCBI
(4003)
Meta
(2434)
RP15
(437)
RP35
(786)
RP55
(1139)
RP75
(1486)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

External links

MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER3.

Pfam alignments:

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Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_1376 (release 3.0) & Pfam-B_5704 (release 7.5)
Previous IDs: UPF0005;
Type: Family
Author: Bateman A
Number in seed: 511
Number in full: 5798
Average length of the domain: 202.60 aa
Average identity of full alignment: 25 %
Average coverage of the sequence by the domain: 84.39 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 26.1 26.1
Trusted cut-off 26.2 26.1
Noise cut-off 25.9 26.0
Model length: 205
Family (HMM) version: 15
Download: download the raw HMM for this family

Species distribution

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