Summary: Alanine dehydrogenase/PNT, C-terminal domain
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Alanine dehydrogenase/PNT, C-terminal domain Provide feedback
This family now also contains the lysine 2-oxoglutarate reductases.
Internal database links
|Similarity to PfamA using HHSearch:||Pyr_redox 2-Hacid_dh_C HI0933_like NAD_binding_2 Shikimate_DH TrkA_N|
External database links
|HOMSTRAD:||AlaDh_PNT_D1 AlaDh_PNT_D2 AlaDh_PNT|
This tab holds annotation information from the InterPro database.
InterPro entry IPR007698
Alanine dehydrogenase catalyses the NAD-dependent reversible reductive amination of pyruvate into alanine. Pyridine nucleotide transhydrogenase catalyses the reduction of NADP+ to NADPH with the concomitant oxidation of NADH to NAD+. This enzyme is located in the plasma membrane of prokaryotes and in the inner membrane of the mitochondria of eukaryotes. The transhydrogenation between NADH and NADP is coupled with the translocation of a proton across the membrane. In prokaryotes the enzyme is composed of two different subunits, an alpha chain (gene pntA) and a beta chain (gene pntB), while in eukaryotes it is a single chain protein.
The sequence of alanine dehydrogenase from several bacterial species is related with that of the alpha subunit of bacterial pyridine nucleotide transhydrogenase and the N-terminal half of the eukaryotic enzyme. The two most conserved regions correspond respectively to the N-terminal domain of these proteins, and to a central glycine-rich region which is part of the NAD(H)-binding site [PUBMED:8439307]. This entry represents a domain found in alanine dehydrogenase and pyridine nucleotide transhydrogenase, containing the glycine-rich region. This domain is also found in lysine 2-oxoglutarate reductases [PUBMED:11354603].
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Curation and family details
|Seed source:||Pfam-B_4166 (release 6.6)|
|Author:||Finn RD, Bateman A|
|Number in seed:||96|
|Number in full:||5257|
|Average length of the domain:||153.50 aa|
|Average identity of full alignment:||35 %|
|Average coverage of the sequence by the domain:||34.75 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||16|
|Download:||download the raw HMM for this family|
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There are 2 interactions for this family. More...
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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the AlaDh_PNT_C domain has been found. There are 97 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.
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