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0  structures 2096  species 0  interactions 4658  sequences 103  architectures

Family: Ferric_reduct (PF01794)

Summary: Ferric reductase like transmembrane component

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Ferric reductase like transmembrane component Provide feedback

This family includes a common region in the transmembrane proteins mammalian cytochrome B-245 heavy chain (gp91-phox), ferric reductase transmembrane component in yeast and respiratory burst oxidase from mouse-ear cress. This may be a family of flavocytochromes capable of moving electrons across the plasma membrane [1]. The Frp1 protein Q04800 from S. pombe is a ferric reductase component and is required for cell surface ferric reductase activity, mutants in frp1 are deficient in ferric iron uptake [1]. Cytochrome B-245 heavy chain P04839 is a FAD-dependent dehydrogenase it is also has electron transferase activity which reduces molecular oxygen to superoxide anion, a precursor in the production of microbicidal oxidants [2]. Mutations in the sequence of cytochrome B-245 heavy chain (gp91-phox) lead to the X-linked chronic granulomatous disease. The bacteriocidal ability of phagocytic cells is reduced and is characterised by the absence of a functional plasma membrane associated NADPH oxidase [3]. The chronic granulomatous disease gene codes for the beta chain of cytochrome B-245 and cytochrome B-245 is missing from patients with the disease [4].

Literature references

  1. Roman DG, Dancis A, Anderson GJ, Klausner RD; , Mol Cell Biol 1993;13:4342-4350.: The fission yeast ferric reductase gene frp1+ is required for ferric iron uptake and encodes a protein that is homologous to the gp91-phox subunit of the human NADPH phagocyte oxidoreductase. PUBMED:8321236 EPMC:8321236

  2. Rotrosen D, Yeung CL, Leto TL, Malech HL, Kwong CH; , Science 1992;256:1459-1462.: Cytochrome b558: the flavin-binding component of the phagocyte NADPH oxidase. PUBMED:1318579 EPMC:1318579

  3. Dinauer MC, Orkin SH, Brown R, Jesaitis AJ, Parkos CA; , Nature 1987;327:717-720.: The glycoprotein encoded by the X-linked chronic granulomatous disease locus is a component of the neutrophil cytochrome b complex. PUBMED:3600768 EPMC:3600768

  4. Teahan C, Rowe P, Parker P, Totty N, Segal AW; , Nature 1987;327:720-721.: The X-linked chronic granulomatous disease gene codes for the beta- chain of cytochrome b-245. PUBMED:3600769 EPMC:3600769


External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR013130

This domain represents a common region in the transmembrane proteins mammalian cytochrome B-245 heavy chain (gp91-phox), ferric reductase transmembrane component in yeast and respiratory burst oxidase from mouse-ear cress. These proteins may be a family of flavocytochromes capable of moving electrons across the plasma membrane [PUBMED:8321236]. The Frp1 protein SWISSPROT from Schizosaccharomyces pombe is a ferric reductase component and is required for cell surface ferric reductase activity. Mutants in Frp1 are deficient in ferric iron uptake [PUBMED:8321236]. Cytochrome B-245 heavy chain SWISSPROT is a FAD-dependent dehydrogenase it is also has electron transferase activity which reduces molecular oxygen to superoxide anion, a precursor in the production of microbicidal oxidants [PUBMED:1318579]. Mutations in the sequence of cytochrome B-245 heavy chain (gp91-phox) lead to the X-linked chronic granulomatous disease. The bacteriocidal ability of phagocytic cells is reduced and is characterised by the absence of a functional plasma membrane associated NADPH oxidase [PUBMED:3600768]. The chronic granulomatous disease gene codes for the beta chain of cytochrome B-245 and cytochrome B-245 is missing from patients with the disease [PUBMED:3600769].

Domain organisation

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Pfam Clan

This family is a member of clan 2heme_cytochrom (CL0328), which has the following description:

This superfamily includes a variety of different heme binding cytochromes.

The clan contains the following 10 members:

COX15-CtaA Cytochrom_B561 Cytochrom_B_N Cytochrom_B_N_2 Cytochrom_C_asm DUF2427 DUF4405 Ferric_reduct Ni_hydr_CYTB PCRF

Alignments

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(209)
Full
(4658)
Representative proteomes NCBI
(3954)
Meta
(660)
RP15
(573)
RP35
(1182)
RP55
(1798)
RP75
(2249)
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1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(209)
Full
(4658)
Representative proteomes NCBI
(3954)
Meta
(660)
RP15
(573)
RP35
(1182)
RP55
(1798)
RP75
(2249)
Alignment:
Format:
Order:
Sequence:
Gaps:
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(209)
Full
(4658)
Representative proteomes NCBI
(3954)
Meta
(660)
RP15
(573)
RP35
(1182)
RP55
(1798)
RP75
(2249)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

External links

MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER3.

Pfam alignments:

HMM logo

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Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_728 (release 4.2)
Previous IDs: none
Type: Family
Author: Bashton M, Bateman A, Finn RD
Number in seed: 209
Number in full: 4658
Average length of the domain: 129.60 aa
Average identity of full alignment: 19 %
Average coverage of the sequence by the domain: 25.54 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 24.3 24.3
Trusted cut-off 24.3 24.3
Noise cut-off 24.1 24.2
Model length: 125
Family (HMM) version: 14
Download: download the raw HMM for this family

Species distribution

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