Summary: Glycosyl hydrolase family 3 C-terminal domain
This is the Wikipedia entry entitled "Glycoside hydrolase family 3". More...
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Glycoside hydrolase family 3 Edit Wikipedia article
|crystal structure of barley beta-d-glucan glucohydrolase isoenzyme exo1|
Glycoside hydrolases EC 3.2.1. are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of >100 different families. This classification is available on the CAZy(http://www.cazy.org/GH1.html) web site, and also discussed at CAZypedia, an online encyclopedia of carbohydrate active enzymes. 
Glycoside hydrolase family 3 CAZY GH_3 comprises enzymes with a number of known activities; beta-glucosidase (EC 22.214.171.124); beta-xylosidase (EC 126.96.36.199); N-acetyl beta-glucosaminidase (EC 188.8.131.52); glucan beta-1,3-glucosidase (EC 184.108.40.206); cellodextrinase (EC 220.127.116.11); exo-1,3-1,4-glucanase (EC 3.2.1). These enzymes are two-domain globular proteins that are N-glycosylated at three sites.
 External references
- ^ Henrissat B, Callebaut I, Mornon JP, Fabrega S, Lehn P, Davies G (1995). "Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases". Proc. Natl. Acad. Sci. U.S.A. 92 (15): 7090–7094. doi:10.1073/pnas.92.15.7090. PMC 41477. PMID 7624375. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=41477.
- ^ Henrissat B, Davies G (1995). "Structures and mechanisms of glycosyl hydrolases". Structure 3 (9): 853–859. doi:10.1016/S0969-2126(01)00220-9. PMID 8535779.
- ^ Bairoch, A. "Classification of glycosyl hydrolase families and index of glycosyl hydrolase entries in SWISS-PROT". 1999.
- ^ Henrissat, B. and Coutinho P.M. "Carbohydrate-Active Enzymes server". 1999.
- ^ CAZypedia, an online encyclopedia of carbohydrate-active enzymes.
- ^ Varghese JN, Fincher GB, Hrmova M (1999). "Three-dimensional structure of a barley beta-D-glucan exohydrolase, a family 3 glycosyl hydrolase". Structure 7 (2): 179–190. doi:10.1016/S0969-2126(99)80024-0. PMID 10368285.
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Glycosyl hydrolase family 3 C-terminal domain Provide feedback
Varghese JN, Hrmova M, Fincher GB; , Structure Fold Des 1999;7:179-190.: Three-dimensional structure of a barley beta-D-glucan exohydrolase, a family 3 glycosyl hydrolase. PUBMED:10368285 EPMC:10368285
External database links
This tab holds annotation information from the InterPro database.
InterPro entry IPR002772
Glycoside hydrolase family 3 CAZY comprises enzymes with a number of known activities; beta-glucosidase (EC); beta-xylosidase (EC); N-acetyl beta-glucosaminidase (EC); glucan beta-1,3-glucosidase (EC); cellodextrinase(EC); exo-1,3-1,4-glucanase (EC).
These enzymes are two-domain globular proteins that are N-glycosylated at three sites [PUBMED:10368285]. This entry represents the C-terminal domain, involved in catalysis and may be involved in binding beta-glucan [PUBMED:10368285]. It is found associated with .
The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.
|Molecular function||hydrolase activity, hydrolyzing O-glycosyl compounds (GO:0004553)|
|Biological process||carbohydrate metabolic process (GO:0005975)|
- the number of sequences which exhibit this architecture
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This example describes an architecture with one
Gladomain, followed by two consecutive
EGFdomains, and finally a single
- the UniProt description of the protein sequence
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We make a range of alignments for each Pfam-A family:
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1Cannot generate PP/Heatmap alignments for seeds; no PP data available
Key: available, not generated, — not available.
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Curation and family details
|Seed source:||Pfam-B_1151 (release 3.0)|
|Author:||Bateman A, Griffiths-Jones SR|
|Number in seed:||107|
|Number in full:||5906|
|Average length of the domain:||251.70 aa|
|Average identity of full alignment:||26 %|
|Average coverage of the sequence by the domain:||33.08 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null --hand HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||17|
|Download:||download the raw HMM for this family|
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There is 1 interaction for this family. More...
We determine these interactions using iPfam, which considers the interactions between residues in three-dimensional protein structures and maps those interactions back to Pfam families. You can find more information about the iPfam algorithm in the journal article that accompanies the website.
For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Glyco_hydro_3_C domain has been found. There are 34 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.
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