Summary: F420-0:Gamma-glutamyl ligase
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F420-0:Gamma-glutamyl ligase Provide feedback
F420-0:Gamma-glutamyl ligase ( EC:6.3.2.-) is an enzyme involved in F420 biosynthesis pathway. It catalyses the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0). This reaction produces polyglutamated F420 derivatives. GTP + F420-0 + n L-glutamate -> GDP + phosphate + F420-n
Nakano T, Miyake K, Endo H, Dairi T, Mizukami T, Katsumata R; , Biosci Biotechnol Biochem. 2004;68:1345-1352.: Identification and cloning of the gene involved in the final step of chlortetracycline biosynthesis in Streptomyces aureofaciens. PUBMED:15215601 EPMC:15215601
Peschke U, Schmidt H, Zhang HZ, Piepersberg W; , Mol Microbiol. 1995;16:1137-1156.: Molecular characterization of the lincomycin-production gene cluster of Streptomyces lincolnensis 78-11. PUBMED:8577249 EPMC:8577249
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This tab holds annotation information from the InterPro database.
InterPro entry IPR002847
This entry contains F420-0:gamma-glutamyl ligase and related proteins. F420-0:gamma-glutamyl ligase catalyzes the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form polyglutamated F420 derivatives [PUBMED:2110564, PUBMED:12867481, PUBMED:8577249, PUBMED:15215601].
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Curation and family details
|Seed source:||Enright A|
|Author:||Enright A, Ouzounis C, Bateman A, MorningStar A, Mistry J|
|Number in seed:||91|
|Number in full:||759|
|Average length of the domain:||231.60 aa|
|Average identity of full alignment:||28 %|
|Average coverage of the sequence by the domain:||69.99 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||11|
|Download:||download the raw HMM for this family|
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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the F420_ligase domain has been found. There are 4 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.
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