Summary: Glycosyl hydrolase family 45
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Glycoside hydrolase family 45 Edit Wikipedia article
|endoglucanase from humicola insolens at 1.7a resolution|
Glycoside hydrolases EC 3.2.1. are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of >100 different families. This classification is available on the CAZy(http://www.cazy.org/GH1.html) web site, and also discussed at CAZypedia, an online encyclopedia of carbohydrate active enzymes.
Glycoside hydrolase family 45 CAZY GH_45 comprises enzymes with only one known activity; endoglucanase (EC 184.108.40.206). This family is also known as cellulase family K. The best conserved region in these enzymes is located in the N-terminal section. It contains an aspartic acid residue which has been shown to act as a nucleophile in the catalytic mechanism. This also has several cysteines that are involved in forming disulphide bridges.
- ^ Henrissat B, Callebaut I, Mornon JP, Fabrega S, Lehn P, Davies G (1995). "Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases". Proc. Natl. Acad. Sci. U.S.A. 92 (15): 7090–7094. doi:10.1073/pnas.92.15.7090. PMC 41477. PMID 7624375. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=41477.
- ^ Henrissat B, Davies G (1995). "Structures and mechanisms of glycosyl hydrolases". Structure 3 (9): 853–859. doi:10.1016/S0969-2126(01)00220-9. PMID 8535779.
- ^ Bairoch, A. "Classification of glycosyl hydrolase families and index of glycosyl hydrolase entries in SWISS-PROT". 1999.
- ^ Henrissat, B. and Coutinho P.M. "Carbohydrate-Active Enzymes server". 1999.
- ^ CAZypedia, an online encyclopedia of carbohydrate-active enzymes.
- ^ Henrissat B, Bairoch A (1993). "New families in the classification of glycosyl hydrolases based on amino acid sequence similarities". Biochem. J. 293: 781–788. PMC 1134435. PMID 8352747. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1134435.
- ^ Dodson GG, Schulein M, Davies GJ, Dauter Z, Wilson KS, Hubbard RE, Tolley SP, Hjort C, Mikkelsen JM, Rasmussen G (1993). "Structure and function of endoglucanase V". Nature 365 (6444): 362–364. doi:10.1038/365362a0. PMID 8377830.
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External database links
This tab holds annotation information from the InterPro database.
InterPro entry IPR000334
O-Glycosyl hydrolases (EC) are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families [PUBMED:7624375, PUBMED:8535779]. This classification is available on the CAZy (CArbohydrate-Active EnZymes) web site.
The microbial degradation of cellulose and xylans requires several types of enzymes such as endoglucanases, cellobiohydrolases (EC) (exoglucanases), or xylanases (EC) [PUBMED:2252383, PUBMED:1886523]. Fungi and bacteria produce a spectrum of cellulolytic enzymes (cellulases) and xylanases which, on the basis of sequence similarities, can be classified into families. One of these families is known as the cellulase family K or as the glycosyl hydrolases family 45 [PUBMED:8352747]. The best conserved regions in these enzymes is located in the N-terminal section. It contains an aspartic acid residue which has been shown [PUBMED:8377830] to act as a nucleophile in the catalytic mechanism. This also has several cysteines that are involved in forming disulphide bridges.
The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.
|Molecular function||cellulase activity (GO:0008810)|
|Biological process||carbohydrate metabolic process (GO:0005975)|
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The DPBB fold is often an enzymatic domain. The members of this family are quite diverse, and if catalytic this family may contain several different functions [1,2]. This clan represents the barwin like barrels.
The clan contains the following 5 members:3D Barwin Cerato-platanin DPBB_1 Glyco_hydro_45
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Curation and family details
|Number in seed:||6|
|Number in full:||228|
|Average length of the domain:||179.70 aa|
|Average identity of full alignment:||43 %|
|Average coverage of the sequence by the domain:||69.24 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||11|
|Download:||download the raw HMM for this family|
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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Glyco_hydro_45 domain has been found. There are 7 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.
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