Summary: Prokaryotic metallothionein
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Prokaryotic metallothionein Provide feedback
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This tab holds annotation information from the InterPro database.
InterPro entry IPR000518
Metallothioneins (MT) are small proteins that bind heavy metals, such as zinc, copper, cadmium and nickel. They have a high content of cysteine residues that bind the metal ions through clusters of thiolate bonds [PUBMED:3064814, PUBMED:2959513, PUBMED:1779825]. An empirical classification into three classes was proposed by Kojima [PUBMED:1779826], with class III MTs including atypical polypeptides composed of gamma-glutamylcysteinyl units. Class I and class II MTs (the proteinaceous sequences) have now been grouped into families of phylogenetically-related and thus alignable sequences. The MT superfamily is subdivided into families, subfamilies, subgroups, and isolated isoforms and alleles. The metallothionein superfamily comprises all polypeptides that resemble equine renal metallothionein in several respects [PUBMED:2959504], e.g., low molecular weight; high metal content; amino acid composition with high Cys and low aromatic residue content; unique sequence with characteristic distribution of cysteines, and spectroscopic manifestations indicative of metal thiolate clusters. A MT family subsumes MTs that share particular sequence-specific features and are thought to be evolutionarily related. Fifteen MT families have been characterised, each family being identified by its number and its taxonomic range.
Family 14 consists of prokaryota MTs. Its members are recognised by the sequence pattern K-C-A-C-x(2)-C-L-C.The taxonomic range of the members extends to cyanobacteria. Known characteristics are: 53 to 56 AAs; 9 conserved Cys; one conserved tyrosine residue; one conserved histidine residue; contain other unusual residues.
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|Molecular function||metal ion binding (GO:0046872)|
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This superfamily contains related families of metallothioneins, prokaryotes and eukaryotes.
The clan contains the following 5 members:Metallothi_Euk2 Metallothio Metallothio_6 Metallothio_Euk Metallothio_Pro
We make a range of alignments for each Pfam-A family:
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Curation and family details
|Author:||Mian N, Bateman A|
|Number in seed:||13|
|Number in full:||100|
|Average length of the domain:||47.90 aa|
|Average identity of full alignment:||40 %|
|Average coverage of the sequence by the domain:||73.21 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||11|
|Download:||download the raw HMM for this family|
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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Metallothio_Pro domain has been found. There are 1 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.
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