Please note: this site relies heavily on the use of javascript. Without a javascript-enabled browser, this site will not function correctly. Please enable javascript and reload the page, or switch to a different browser.
47  structures 359  species 8  interactions 1878  sequences 196  architectures

Family: HEAT (PF02985)

Summary: HEAT repeat

Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.

This is the Wikipedia entry entitled "HEAT repeat domain". More...

HEAT repeat domain Edit Wikipedia article

HEAT repeat
PDB 1b3u EBI.jpg
Structure of the protein phosphatase 2A PR65/A subunit revealing the conformation of its 15 tandemly repeated HEAT motifs.[1]
Identifiers
Symbol HEAT
Pfam PF02985
InterPro IPR000357
PROSITE PDOC50077
SCOP 1b3u
SUPERFAMILY 1b3u

The HEAT repeat domain is a solenoid protein domain found in a number of cytoplasmic proteins including the four that the give rise to the acronym HEAT (Huntingtin, elongation factor 3 (EF3), protein phosphatase 2A (PP2A), and the yeast kinase TOR1).[2] HEAT repeats form a rod-like helical structures which are involved in intracellular transport.

References[edit]

  1. ^ Groves MR, Hanlon N, Turowski P, Hemmings BA, Barford D (January 1999). "The structure of the protein phosphatase 2A PR65/A subunit reveals the conformation of its 15 tandemly repeated HEAT motifs". Cell 96 (1): 99–110. doi:10.1016/S0092-8674(00)80963-0. PMID 9989501. 
  2. ^ Andrade MA, Bork P (October 1995). "HEAT repeats in the Huntington's disease protein". Nat. Genet. 11 (2): 115–6. doi:10.1038/ng1095-115. PMID 7550332. 

External links[edit]


This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

HEAT repeat Provide feedback

The HEAT repeat family is related to armadillo/beta-catenin-like repeats (see PF00514).

Literature references

  1. Andrade MA, Bork P; , Nat Genet 1995;11:115-116.: HEAT repeats in the Huntington's disease protein. PUBMED:7550332 EPMC:7550332

  2. Groves MR, Hanlon N, Turowski P, Hemmings BA, Barford D; , Cell 1999;96:99-110.: The structure of the protein phosphatase 2A PR65/A subunit reveals the conformation of its 15 tandemly repeated HEAT motifs. PUBMED:9989501 EPMC:9989501


Internal database links

External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR000357

The HEAT repeat is a tandemly repeated, 37-47 amino acid long module occurring in a number of cytoplasmic proteins, including the four name-giving proteins huntingtin, elongation factor 3 (EF3), the 65 Kd alpha regulatory subunit of protein phosphatase 2A (PP2A) and the yeast PI3-kinase TOR1 [PUBMED:7550332]. Arrays of HEAT repeats consists of 3 to 36 units forming a rod-like helical structure and appear to function as protein-protein interaction surfaces. It has been noted that many HEAT repeat-containing proteins are involved in intracellular transport processes.

In the crystal structure of PP2A PR65/A [PUBMED:9989501], the HEAT repeats consist of pairs of antiparallel alpha helices [PUBMED:7550332].

Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

Loading domain graphics...

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the NCBI sequence database, and our metagenomics sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(590)
Full
(1878)
Representative proteomes NCBI
(5988)
Meta
(309)
RP15
(419)
RP35
(626)
RP55
(897)
RP75
(1138)
Jalview View  View  View  View  View  View  View  View 
HTML View  View  View  View  View  View     
PP/heatmap 1 View  View  View  View  View     
Pfam viewer View  View             

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(590)
Full
(1878)
Representative proteomes NCBI
(5988)
Meta
(309)
RP15
(419)
RP35
(626)
RP55
(897)
RP75
(1138)
Alignment:
Format:
Order:
Sequence:
Gaps:
Download/view:

Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(590)
Full
(1878)
Representative proteomes NCBI
(5988)
Meta
(309)
RP15
(419)
RP35
(626)
RP55
(897)
RP75
(1138)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

External links

MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER3.

Pfam alignments:

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Reference [2]
Previous IDs: none
Type: Repeat
Author: Griffiths-Jones SR
Number in seed: 590
Number in full: 1878
Average length of the domain: 30.50 aa
Average identity of full alignment: 27 %
Average coverage of the sequence by the domain: 3.66 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 23.5 17.6
Trusted cut-off 23.5 17.6
Noise cut-off 23.4 17.5
Model length: 31
Family (HMM) version: 17
Download: download the raw HMM for this family

Species distribution

Sunburst controls

Show

This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

Loading sunburst data...

Tree controls

Hide

The tree shows the occurrence of this domain across different species. More...

Loading...

Please note: for large trees this can take some time. While the tree is loading, you can safely switch away from this tab but if you browse away from the family page entirely, the tree will not be loaded.

Interactions

There are 8 interactions for this family. More...

Arm Cullin Ras IBN_N HnRNP_M HEAT Metallophos Cullin_Nedd8

Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the HEAT domain has been found. There are 47 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.

Loading structure mapping...