Summary
Outer membrane protein transport protein (OMPP1/FadL/TodX)
This family includes TodX from Pseudomonas putida F1 Q51971 and TbuX from Ralstonia pickettii PKO1 Q9RBW8. These are membrane proteins of uncertain function that are involved in toluene catabolism. Related proteins involved in the degradation of similar aromatic hydrocarbons are also in this family, such as CymD O33458. This family also includes FadL involved in translocation of long-chain fatty acids across the outer membrane. It is also a receptor for the bacteriophage T2.
Literature references
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Wang Y, Rawlings M, Gibson DT, Labbe D, Bergeron H, Brousseau R, Lau PC; , Mol Gen Genet 1995;246:570-579.: Identification of a membrane protein and a truncated LysR-type regulator associated with the toluene degradation pathway in Pseudomonas putida F1. PUBMED:7535376
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Kahng HY, Byrne AM, Olsen RH, Kukor JJ; , J Bacteriol 2000;182:1232-1242.: Characterization and role of tbuX in utilization of toluene by Ralstonia pickettii PKO1. PUBMED:10671442
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Eaton RW; , J Bacteriol 1997;179:3171-3180.: p-Cymene catabolic pathway in Pseudomonas putida F1: cloning and characterization of DNA encoding conversion of p-cymene to p-cumate. PUBMED:9150211
InterPro entry IPR005017
This family includes TodX from Pseudomonas putida (strain F1/ATCC 700007) and TbuX from Burkholderia pickettii (Ralstonia pickettii) (Pseudomonas pickettii) PKO1 . These are membrane proteins of uncertain function that are involved in toluene catabolism. Related proteins involved in the degradation of similar aromatic hydrocarbons are also in this family, such as CymD .
Clan
This family is a member of clan MBB (CL0193), which contains the following 36 members:
Ail_Lom Autotransporter Bac_surface_Ag Channel_Tsx CopB DUF1302 DUF2320 DUF2860 DUF3078 DUF3575 DUF481 DUF560 Gcw_chp HP_OMP KdgM MipA OmpA_membrane Omptin OmpW Opacity OpcA OprB OprD OprF OstA_C PagL Porin_1 Porin_2 Porin_O_P Porin_OmpG ShlB Surface_Ag_2 Toluene_X TonB_dep_Rec Usher YfaZInternal database links
| SCOOP: | DUF2320 |
External database links
| PANDIT: | PF03349 |
| SYSTERS: | Toluene_X |
| Transporter classification: | 1.B.9 |
Domain organisation
Below is a listing of the unique domain organisations or architectures in which this domain is found. More...
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Alignments
There are various ways to view or download the sequence alignments that we store. You can use a sequence viewer to look at either the seed or full alignment for the family, or you can look at a plain text version of the sequence in a variety of different formats. More...
View options
Formatting options
Download options
Very large alignments can often cause problems for the formatting tool above. If you find that downloading or viewing a large alignment is problematic, you can also download a gzip-compressed, Stockholm-format file containing the seed or full alignment for this family.
You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.
The main seed and full alignments are generated using sequences from the UniProt sequence database. However, we also generate alignments using sequences from the NCBI sequence database and the "metaseq" metagenomics dataset.
You can view alignments from these two additional datasets using the form above, or you can download alignments of NCBI or metagenomics sequences, as gzip-compressed files.
External links
MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER2.
HMM logo
HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...
Trees
This page displays the phylogenetic tree for this family. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed or full alignments.
Note: You can also download the data files for the seed, full, NCBI or metagenomics trees.
Curation and family details
This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.
Curation
| Seed source: | Pfam-B_3708 (release 6.5) |
| Previous IDs: | none |
| Type: | Family |
| Author: | Mifsud W |
| Number in seed: | 22 |
| Number in full: | 1074 |
| Average length of the domain: | 393.00 aa |
| Average identity of full alignment: | 20 % |
| Average coverage of the sequence by the domain: | 91.47 % |
HMM information
| HMM build commands: |
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 9421015 -E 1000 HMM pfamseq
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| Model details: |
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| Model length: | 427 | ||||||||||||
| Family (HMM) version: | 9 | ||||||||||||
| Download: | download the raw HMM for this family |
Species distribution
Tree controls
HideThe tree shows the occurrence of this domain across different species. More...
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Structures
For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Toluene_X domain has been found.
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