Summary: Arginine-tRNA-protein transferase, N terminus
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Arginine-tRNA-protein transferase, N terminus Provide feedback
This family represents the N terminal region of the enzyme arginine-tRNA-protein transferase (EC 22.214.171.124), which catalyses the post-translational conjugation of arginine to the N terminus of a protein. In eukaryotes, this functions as part of the N-end rule pathway of protein degradation by conjugating a de-stabilising amino acid to the amino terminal aspartate or glutamate of a protein, targeting the protein for ubiquitin-dependent proteolysis. N terminal cysteine is sometimes modified . In S cerevisiae, Cys20, 23, 94 and/or 95 are thought to be important for activity . Of these, only Cys 94 appears to be completely conserved in this family.
Kwon YT, Kashina AS, Varshavsky A; , Mol Cell Biol 1999;19:182-193.: Alternative splicing results in differential expression, activity, and localization of the two forms of arginyl-tRNA-protein transferase, a component of the N-end rule pathway. PUBMED:9858543 EPMC:9858543
External database links
This tab holds annotation information from the InterPro database.
InterPro entry IPR007471
Arginine-tRNA-protein transferase catalyses the post-translational conjugation of arginine to the N terminus of a protein. In eukaryotes, this functions as part of the N terminus rule pathway of protein degradation by conjugating a destabilising amino acid to the N-terminal aspartate or glutamate of a protein, targeting the protein for ubiquitin-dependent proteolysis. N-terminal cysteine is sometimes modified [PUBMED:9858543]. In Saccharomyces cerevisiae, Cys20, 23, 94 and/or 95 are thought to be important for activity [PUBMED:7495814]. Of these, only Cys 94 appears to be completely conserved in this family.
This entry represents the N-terminal region of the enzyme arginine-tRNA-protein transferase, found in both eukaryotic and prokaryotic enzymes.
The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.
|Molecular function||arginyltransferase activity (GO:0004057)|
|Biological process||protein arginylation (GO:0016598)|
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This clan contains families related to N-acetyltransferases. N-acetyltransferases catalyse the transfer of acetyl groups from acetyl-CoA to arylamines.
The clan contains the following 31 members:Acetyltransf_1 Acetyltransf_10 Acetyltransf_13 Acetyltransf_3 Acetyltransf_4 Acetyltransf_5 Acetyltransf_6 Acetyltransf_7 Acetyltransf_8 Acetyltransf_9 Acetyltransf_CG ATE_C ATE_N Autoind_synth DUF1248 DUF1999 DUF2156 DUF3749 DUF482 DUF619 FemAB FR47 Gly_acyl_tr_C GNAT_acetyltr_2 GNAT_acetyltran Leu_Phe_trans Mec-17 Mig-14 MOZ_SAS NMT NodA
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Curation and family details
|Number in seed:||128|
|Number in full:||1240|
|Average length of the domain:||77.30 aa|
|Average identity of full alignment:||35 %|
|Average coverage of the sequence by the domain:||25.38 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||8|
|Download:||download the raw HMM for this family|
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