Summary: Phage Connector (GP10)
Phage Connector (GP10) Provide feedback
The head-tail connector of bacteriophage 29 is composed of 12 36 kDa subunits with 12 fold symmetry. It is the central component of a rotary motor that packages the genomic dsDNA into pre-formed proheads. This motor consists of the head-tail connector, surrounded by a 29-encoded, 174-base, RNA and a viral ATPase protein [1,2].
Simpson AA, Leiman PG, Tao Y, He Y, Badasso MO, Jardine PJ, Anderson DL, Rossmann MG; , Acta Crystallogr D Biol Crystallogr 2001;57:1260-1269.: Structure determination of the head-tail connector of bacteriophage phi29. PUBMED:11526317 EPMC:11526317
Simpson AA, Tao Y, Leiman PG, Badasso MO, He Y, Jardine PJ, Olson NH, Morais MC, Grimes S, Anderson DL, Baker TS, Rossmann MG; , Nature 2000;408:745-750.: Structure of the bacteriophage phi29 DNA packaging motor. PUBMED:11130079 EPMC:11130079
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This tab holds annotation information from the InterPro database.
InterPro entry IPR008016
This entry represents the upper collar protein (also known as head-tail connector protein or late protein Gp10) from various bacteriophage. The upper collar protein of Bacteriophage phi-29 is composed of twelve 36 kDa subunits with 12-fold symmetry. It consists of two domains: an alpha-helical bundle domain and a beta-barrel domain. This protein is located between the head and the tail of the bacteriophage and acts as the central component of a rotary motor that packages the genomic dsDNA into pre-formed proheads. This motor consists of the upper collar protein, surrounded by a 29-encoded, 174-base, RNA and a viral ATPase protein [PUBMED:9891587].
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Curation and family details
|Seed source:||Pfam-B_13828 (release 7.8)|
|Number in seed:||4|
|Number in full:||23|
|Average length of the domain:||271.50 aa|
|Average identity of full alignment:||30 %|
|Average coverage of the sequence by the domain:||88.42 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||7|
|Download:||download the raw HMM for this family|
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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Phage_connector domain has been found. There are 39 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.
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