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59  structures 1933  species 2  interactions 3225  sequences 49  architectures

Family: Glyco_hydro_32C (PF08244)

Summary: Glycosyl hydrolases family 32 C terminal

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This is the Wikipedia entry entitled "Glycoside hydrolase family 32". More...

Glycoside hydrolase family 32 Edit Wikipedia article

Glycosyl hydrolases family 32 N-terminal domain
PDB 1w2t EBI.jpg
beta-fructosidase from thermotoga maritima in complex with raffinose
Identifiers
Symbol Glyco_hydro_32N
Pfam PF00251
Pfam clan CL0143
InterPro IPR013148
PROSITE PDOC00532
CAZy GH32
Glycosyl hydrolases family 32 C terminal
PDB 1y4w EBI.jpg
crystal structure of exo-inulinase from aspergillus awamori in spacegroup p21
Identifiers
Symbol Glyco_hydro_32C
Pfam PF08244
InterPro IPR013189
PROSITE PDOC00532
CAZy GH32

In molecular biology, glycoside hydrolase family 32 is a family of glycoside hydrolases.

Glycoside hydrolases EC 3.2.1. are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of >100 different families.[1][2][3] This classification is available on the CAZy(http://www.cazy.org/GH1.html) web site,[4] and also discussed at CAZypedia, an online encyclopedia of carbohydrate active enzymes.[5]

Family 32 glycosyl hydrolases comprise two distinct domains. The N-terminal domain, which forms a five bladed beta propeller, and the C-terminal domain, which forms a beta sandwich structure.[6]

References[edit]

This article incorporates text from the public domain Pfam and InterPro IPR013189

This article incorporates text from the public domain Pfam and InterPro IPR013148

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Glycosyl hydrolases family 32 C terminal Provide feedback

This domain corresponds to the C terminal domain of glycosyl hydrolase family 32. It forms a beta sandwich module [1].

Literature references

  1. Alberto F, Bignon C, Sulzenbacher G, Henrissat B, Czjzek M; , J Biol Chem 2004;279:18903-18910.: The three-dimensional structure of invertase (beta-fructosidase) from Thermotoga maritima reveals a bimodular arrangement and an evolutionary relationship between retaining and inverting glycosidases. PUBMED:14973124 EPMC:14973124


External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR013189

This domain corresponds to the C-terminal domain of glycosyl hydrolase family 32. It forms a beta sandwich module [PUBMED:14973124].

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the NCBI sequence database, and our metagenomics sequence database. More...

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(178)
Full
(3225)
Representative proteomes NCBI
(2522)
Meta
(88)
RP15
(129)
RP35
(322)
RP55
(462)
RP75
(570)
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Format an alignment

  Seed
(178)
Full
(3225)
Representative proteomes NCBI
(2522)
Meta
(88)
RP15
(129)
RP35
(322)
RP55
(462)
RP75
(570)
Alignment:
Format:
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Sequence:
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(178)
Full
(3225)
Representative proteomes NCBI
(2522)
Meta
(88)
RP15
(129)
RP35
(322)
RP55
(462)
RP75
(570)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

External links

MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER3.

Pfam alignments:

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Prosite
Previous IDs: glycosyl_hydro2; Glyco_hydro_32;
Type: Domain
Author: Finn RD, Mistry J
Number in seed: 178
Number in full: 3225
Average length of the domain: 88.70 aa
Average identity of full alignment: 22 %
Average coverage of the sequence by the domain: 16.56 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 21.3 21.3
Trusted cut-off 21.3 21.3
Noise cut-off 21.2 21.2
Model length: 86
Family (HMM) version: 7
Download: download the raw HMM for this family

Species distribution

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Interactions

There are 2 interactions for this family. More...

Glyco_hydro_32C Glyco_hydro_32N

Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Glyco_hydro_32C domain has been found. There are 59 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.

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