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0  structures 3  species 0  interactions 5  sequences 1  architecture

Family: Mastoparan_2 (PF08251)

Summary: Mastoparan peptide

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This is the Wikipedia entry entitled "Mastoparan". More...

Mastoparan Edit Wikipedia article

Mastoparan
Mastoparan.png
Solution structure of mastoparan from Vespa simillima xanthoptera.[1]
Identifiers
Symbol Mastoparan_2
Pfam PF08251
InterPro IPR013214
TCDB 1.C.32
OPM superfamily 160
OPM protein 2czp

Mastoparan is a peptide toxin from wasp venom. It has the chemical structure Ile-Asn-Leu-Lys-Ala-Leu-Ala-Ala-Leu-Ala-Lys-Lys-Ile-Leu-NH2.[2]

The net effect of mastoparan's mode of action depends on cell type, but seemingly always involves exocytosis. In mast cells, this takes the form of histamine secretion, while in platelets and chromaffin cells release serotonin and catecholamines are found, respectively. Mastoporan activity in the anterior pituitary gland leads to prolactin release.

In the case of histamine secretion, the effect of mastoparan takes place via its interference with G protein activity. By stimulating the GTPase activity of certain subunits, mastoparan shortens the lifespan of active G protein. At the same time, it promotes dissociation of any bound GDP from the protein, enhancing GTP binding. In effect, the GTP turnover of G proteins is greatly increased by mastoparan. These properties of the toxin follow from the fact that it structurally resembles activated G protein receptors when placed in a phospholipid environment. The resultant G protein-mediated signaling cascade leads to intracellular IP3 release and the resultant influx of Ca2+.

In an experimental study conducted by Tsutomu Higashijima and his counterparts, mastoparan was compared to melittin, which is found in bee venom.[2] Mainly, the structure and reaction to phosphate was studied in each toxin. Using Circular Dichroism (CD), it was found that when mastoparan was exposed to methanol, an alpha helical form existed. It was concluded that strong intramolecular hydrogen bonding occurred. Also, two negative bands were present on the CD spectrum. In an aqueous environment, mastoparan took on a nonhelical, unordered form. In this case, only one negative band was observed on the CD spectrum. Adding phosphate buffer to mastoparan resulted in no effect.

Melittin produced a different conformational change than mastoparan. In an aqueous solution, melittin went from a nonhelical form to an alpha helix when phosphate was added to the solution. The binding of melittin to the membrane was believed to result from electrostatic interactions, not hydrophobic interactions.

References[edit]

  1. ^ PDB 2CZP; Todokoro Y, Yumen I, Fukushima K, Kang SW, Park JS, Kohno T, Wakamatsu K, Akutsu H, Fujiwara T (August 2006). "Structure of Tightly Membrane-Bound Mastoparan-X, a G-Protein-Activating Peptide, Determined by Solid-State NMR". Biophys. J. 91 (4): 1368–79. doi:10.1529/biophysj.106.082735. PMC 1518647. PMID 16714348. 
  2. ^ a b Higashijima T, Uzu S, Nakajima T, Ross EM (May 1988). "Mastoparan, a peptide toxin from wasp venom, mimics receptors by activating GTP-binding regulatory proteins (G proteins)". J. Biol. Chem. 263 (14): 6491–4. PMID 3129426. 

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This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Mastoparan peptide Provide feedback

Mastoparan (MP) peptides I II and III are extracted from the venom gland of the Neotropical social wasp Protopolybia exigua(Saussure) They are tetradecapeptides presenting from seven to ten hydrophobic amino acid residues and from two to four lysine residues in their primary sequences. These peptide cause the degranulation of mast cells. Protopolybia-MP-I also act causing hemolysis in erythrocytes.

Literature references

  1. Mendes MA, de Souza BM, Palma MS; , Toxicon 2005;45:101-106.: Structural and biological characterization of three novel mastoparan peptides from the venom of the neotropical social wasp Protopolybia exigua (Saussure). PUBMED:15581688 EPMC:15581688


External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR013214

Mastoparan (MP) peptides I, II and III are extracted from the venom gland of Protopolybia exigua (Neotropical social wasp). They are tetradecapeptides presenting from seven to ten hydrophobic amino acid residues and from two to four lysine residues in their primary sequences. These peptides cause the degranulation of mast cells. Protopolybia-MP-I also causes haemolysis of erythrocytes.

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Seed source: Short protein clustering
Previous IDs: none
Type: Family
Author: Rossi R
Number in seed: 2
Number in full: 5
Average length of the domain: 14.00 aa
Average identity of full alignment: 76 %
Average coverage of the sequence by the domain: 100.00 %

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Parameter Sequence Domain
Gathering cut-off 25.0 25.0
Trusted cut-off 26.1 26.1
Noise cut-off 16.5 15.0
Model length: 14
Family (HMM) version: 6
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