Summary: nsp8 replicase
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Viral nsp8 (non structural protein 8) forms a hexadecameric supercomplex with nsp7 that adopts a hollow cylinder-like structure . The dimensions of the central channel and positive electrostatic properties of the cylinder imply that it confers processivity on RNA-dependent RNA polymerase .
Zhai Y, Sun F, Li X, Pang H, Xu X, Bartlam M, Rao Z; , Nat Struct Mol Biol. 2005;12:980-986.: Insights into SARS-CoV transcription and replication from the structure of the nsp7-nsp8 hexadecamer. PUBMED:16228002 EPMC:16228002
External database links
This tab holds annotation information from the InterPro database.
InterPro entry IPR014829
Viral Nsp8 (non structural protein 8) forms a hexadecameric supercomplex with Nsp7 that adopts a hollow cylinder-like structure [PUBMED:16228002]. The dimensions of the central channel and positive electrostatic properties of the cylinder imply that it confers processivity on RNA-dependent RNA polymerase [PUBMED:16228002].
The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.
|Molecular function||cysteine-type endopeptidase activity (GO:0004197)|
|transferase activity (GO:0016740)|
|omega peptidase activity (GO:0008242)|
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Curation and family details
|Number in seed:||9|
|Number in full:||574|
|Average length of the domain:||198.40 aa|
|Average identity of full alignment:||54 %|
|Average coverage of the sequence by the domain:||3.56 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||5|
|Download:||download the raw HMM for this family|
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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the nsp8 domain has been found. There are 6 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.
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