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2  structures 26  species 0  interactions 55  sequences 2  architectures

Family: Kp4 (PF09044)

Summary: Kp4

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This is the Wikipedia entry entitled "Killer toxin Kp4 family". More...

Killer toxin Kp4 family Edit Wikipedia article

Kp4
PDB 1kpt EBI.jpg
structure and function of a virally encoded fungal toxin from ustilago maydis: a fungal and mammalian calcium channel inhibitor
Identifiers
Symbol Kp4
Pfam PF09044
InterPro IPR015131

In molelcular biology, the killer toxin Kp4 family is a family of killer toxins, which includes the Kp4 killer toxin from the smut fungus Ustilago maydis.[1]

Killer toxins are polypeptides secreted by some fungal species that kill sensitive cells of the same or related species, often functioning by creating pores in target cell membranes. The fungal killer toxin KP4 from the corn smut fungus, Ustilago maydis (Smut fungus), is encoded by a resident symbiotic double-stranded RNA virus, Ustilago maydis P4 virus (UmV4), within fungal cells. Unlike most killer toxins, KP4 is a single polypeptide.[1] KP4 inhibits voltage-gated calcium channels in mammalian cells, which in turn inhibits cell growth and division by blocking calcium import. KP4 adopts a structure consisting of a two-layer alpha/beta sandwich with a left-handed crossover.[2]


References[edit]

  1. ^ a b Park CM, Bruenn JA, Ganesa C, Flurkey WF, Bozarth RF, Koltin Y (January 1994). "Structure and heterologous expression of the Ustilago maydis viral toxin KP4". Mol. Microbiol. 11 (1): 155–64. PMID 8145639. 
  2. ^ Gu F, Khimani A, Rane SG, Flurkey WH, Bozarth RF, Smith TJ (August 1995). "Structure and function of a virally encoded fungal toxin from Ustilago maydis: a fungal and mammalian Ca2+ channel inhibitor". Structure 3 (8): 805–14. PMID 7582897. 

This article incorporates text from the public domain Pfam and InterPro IPR015131

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

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Members of this fungal family of toxins specifically inhibit voltage-gated calcium channels in mammalian cells. They adopt an alpha/beta-sandwich structure, comprising a five-stranded antiparallel beta-sheet with two antiparallel alpha-helices lying at approximately 45 degrees to these strands [1].

Literature references

  1. Gu F, Khimani A, Rane SG, Flurkey WH, Bozarth RF, Smith TJ; , Structure. 1995;3:805-814.: Structure and function of a virally encoded fungal toxin from Ustilago maydis: a fungal and mammalian Ca2+ channel inhibitor. PUBMED:7582897 EPMC:7582897


External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR015131

Killer toxins are polypeptides secreted by some fungal species that kill sensitive cells of the same or related species, often functioning by creating pores in target cell membranes. The fungal killer toxin KP4 from the corn smut fungus, Ustilago maydis (Smut fungus), is encoded by a resident symbiotic double-stranded RNA virus, Ustilago maydis P4 virus (UmV4), within fungal cells. Unlike most killer toxins, KP4 is a single polypeptide [PUBMED:8145639]. KP4 inhibits voltage-gated calcium channels in mammalian cells, which in turn inhibits cell growth and division by blocking calcium import. KP4 adopts a structure consisting of a two-layer alpha/beta sandwich with a left-handed crossover [PUBMED:7582897].

Domain organisation

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Alignments

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(2)
Full
(55)
Representative proteomes NCBI
(54)
Meta
(0)
RP15
(7)
RP35
(24)
RP55
(37)
RP75
(49)
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  Seed
(2)
Full
(55)
Representative proteomes NCBI
(54)
Meta
(0)
RP15
(7)
RP35
(24)
RP55
(37)
RP75
(49)
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You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

External links

MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER3.

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This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

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Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

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Seed source: pdb_1kpt
Previous IDs: none
Type: Domain
Author: Mistry J, Sammut SJ
Number in seed: 2
Number in full: 55
Average length of the domain: 118.20 aa
Average identity of full alignment: 34 %
Average coverage of the sequence by the domain: 83.92 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 25.0 25.0
Trusted cut-off 26.3 26.2
Noise cut-off 24.3 24.3
Model length: 128
Family (HMM) version: 5
Download: download the raw HMM for this family

Species distribution

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Kp4 domain has been found. There are 2 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.

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