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0  structures 148  species 0  interactions 202  sequences 3  architectures

Family: Kua-UEV1_localn (PF10520)

Summary: Kua-ubiquitin conjugating enzyme hybrid localisation domain

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Kua-ubiquitin conjugating enzyme hybrid localisation domain Provide feedback

This domain is part of the transcript of the fusion of two genes, the UEV1, an enzymatically inactive variant of the E2 ubiquitin-conjugating enzymes that regulate non-canonical elongation of ubiquitin chains, and Kua, an otherwise unknown gene. UEV1A is a nuclear protein, whereas both Kua and Kua-UEV localise to cytoplasmic structures, indicating that the addition of a Kua domain to UEV confers new biological properties. UEV1-Kua carries the B domain with its characteristic double histidine motif, and it is probably this domain which determines the cytoplasmic localisation. It is postulated that this hybrid transcript could preferentially direct the variant polyubiquitination of substrates closely associated with the cytoplasmic face of the endoplasmic reticulum, possibly, although not necessarily, in conjunction with membrane-bound ubiquitin-conjugating enzymes [1].

Literature references

  1. Thomson TM, Lozano JJ, Loukili N, Carrio R, Serras F, Cormand B, Valeri M, Diaz VM, Abril J, Burset M, Merino J, Macaya A, Corominas M, Guigo R; , Genome Res. 2000;10:1743-1756.: Fusion of the human gene for the polyubiquitination coeffector UEV1 with Kua, a newly identified gene. PUBMED:11076860 EPMC:11076860


External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR019547

This entry represents part of the transcript of the fusion of two genes, the UEV1.

UEV1 is an enzymatically inactive variant of the E2 ubiquitin-conjugating enzymes that regulate non-canonical elongation of ubiquitin chains, and Kua, an otherwise unknown gene. UEV1A is a nuclear protein, whereas both Kua and Kua-UEV localise to cytoplasmic structures, indicating that the addition of a Kua domain to UEV confers new biological properties. UEV1-Kua carries the B domain with its characteristic double histidine motif, and it is probably this domain which determines the cytoplasmic localisation. It is postulated that this hybrid transcript could preferentially direct the variant polyubiquitination of substrates closely associated with the cytoplasmic face of the endoplasmic reticulum, possibly, although not necessarily, in conjunction with membrane-bound ubiquitin-conjugating enzymes [PUBMED:11076860].

Domain organisation

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Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the NCBI sequence database, and our metagenomics sequence database. More...

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(21)
Full
(202)
Representative proteomes NCBI
(190)
Meta
(17)
RP15
(45)
RP35
(66)
RP55
(98)
RP75
(125)
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1Cannot generate PP/Heatmap alignments for seeds; no PP data available

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Format an alignment

  Seed
(21)
Full
(202)
Representative proteomes NCBI
(190)
Meta
(17)
RP15
(45)
RP35
(66)
RP55
(98)
RP75
(125)
Alignment:
Format:
Order:
Sequence:
Gaps:
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(21)
Full
(202)
Representative proteomes NCBI
(190)
Meta
(17)
RP15
(45)
RP35
(66)
RP55
(98)
RP75
(125)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download   Download  
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You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

External links

MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER3.

Pfam alignments:

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: TreeFam_TF106147
Previous IDs: none
Type: Domain
Author: Buljan M, Coggill P
Number in seed: 21
Number in full: 202
Average length of the domain: 167.40 aa
Average identity of full alignment: 46 %
Average coverage of the sequence by the domain: 60.55 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 21.7 21.7
Trusted cut-off 24.3 23.4
Noise cut-off 20.3 19.5
Model length: 178
Family (HMM) version: 4
Download: download the raw HMM for this family

Species distribution

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