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0  structures 225  species 0  interactions 270  sequences 8  architectures

Family: Misat_Tub_SegII (PF10644)

Summary: Misato Segment II tubulin-like domain

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Misato Segment II tubulin-like domain Provide feedback

The misato protein contains three distinct, conserved domains, segments I, II and III. Segments I and III are common to Tubulins PF00091 but segment II aligns with myosin heavy chain sequences from D. melanogaster (PIR C35815), rabbit (SP P04460), and human (PIR S12458). Segment II of misato is a major contributor to its greater length compared with the various tubulins. The most significant sequence similarities to this 54-amino acid region are from a motif found in the heavy chains of myosins from different organisms. A comparison of segment II with the vertebrate myosin heavy chains reveals that it is homologous to a myosin peptide in the hinge region linking the S2 and LMM domains. Segment II also contains heptad repeats which are characteristic of the myosin tail alpha-helical coiled-coils [1]. This myosin-like homology may be due only to the fact that both myosin and Misato carry coiled-coils, which appear similar but are not necessarily homologous (Wood V, personal communication).

Literature references

  1. Miklos GL, Yamamoto M, Burns RG, Maleszka R; , Proc Natl Acad Sci U S A. 1997;94:5189-5194.: An essential cell division gene of Drosophila, absent from Saccharomyces, encodes an unusual protein with tubulin-like and myosin-like peptide motifs. PUBMED:9144213 EPMC:9144213


External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR019605

The misato protein contains three distinct, conserved domains, segments I, II and III and is involved in the regulation of mitochondrial distribution and morphology [PUBMED:17349998]. This entry represents misato segment II.

Segments I and III are common to tubulins (INTERPRO), but segment II aligns with myosin heavy chain sequences from Drosophila melanogaster (Fruit fly, SWISSPROT), rabbit (SWISSPROT), and human.

Segment II of misato is a major contributor to its greater length compared with the various tubulins. The most significant sequence similarities to this 54-amino acid region are from a motif found in the heavy chains of myosins from different organisms. A comparison of segment II with the vertebrate myosin heavy chains reveals that it is homologous to a myosin peptide in the hinge region linking the S2 and LMM domains. Segment II also contains heptad repeats which are characteristic of the myosin tail alpha-helical coiled-coils [PUBMED:9144213].

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan Tubulin-like (CL0442), which has the following description:

This superfamily is characterised by being of alpha-beta two-layer sandwich structures. A segmental duplication early on in the primate lineage has led to the creation of up to three tubulin-like domains in the higher primates. The most N-terminal one is the Misato domain, which probably represents the original domain [3].

The clan contains the following 6 members:

FtsZ_C Misat_Tub_SegII Tubulin Tubulin_2 Tubulin_3 Tubulin_C

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the NCBI sequence database, and our metagenomics sequence database. More...

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(51)
Full
(270)
Representative proteomes NCBI
(2002)
Meta
(14)
RP15
(53)
RP35
(98)
RP55
(150)
RP75
(187)
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1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(51)
Full
(270)
Representative proteomes NCBI
(2002)
Meta
(14)
RP15
(53)
RP35
(98)
RP55
(150)
RP75
(187)
Alignment:
Format:
Order:
Sequence:
Gaps:
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Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(51)
Full
(270)
Representative proteomes NCBI
(2002)
Meta
(14)
RP15
(53)
RP35
(98)
RP55
(150)
RP75
(187)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

External links

MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER3.

Pfam alignments:

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_7826 (release 21.0)
Previous IDs: Misat_Myo_SegII;
Type: Domain
Author: Wood V, Coggill P
Number in seed: 51
Number in full: 270
Average length of the domain: 111.20 aa
Average identity of full alignment: 33 %
Average coverage of the sequence by the domain: 21.93 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 21.5 21.5
Trusted cut-off 21.5 21.5
Noise cut-off 21.4 21.4
Model length: 115
Family (HMM) version: 4
Download: download the raw HMM for this family

Species distribution

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