Summary: Endoribonuclease RegB T4-bacteriophage encoded
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Endoribonuclease RegB T4-bacteriophage encoded Provide feedback
The RegB endoribonuclease encoded by bacteriophage T4 is a unique sequence-specific nuclease that cleaves in the middle of GGAG or, in a few cases, GGAU tetranucleotides, preferentially those found in the Shine-Dalgarno regions of early phage mRNAs. Phage RB49 in addition to gpRegB utilises Escherichia coli endoribonuclease E for the degradation of its transcripts for gene regB. The deduced primary structure of RegB proteins of 32 phages studied is almost identical to that of T4, while the sequences of RegB encoded by phages RB69, TuIa and RB49 show substantial divergence from their T4 counterpart.
Piesiniene L, Truncaite L, Zajanckauskaite A, Nivinskas R; , Nucleic Acids Res. 2004;32:5582-5595.: The sequences and activities of RegB endoribonucleases of T4-related bacteriophages. PUBMED:15486207 EPMC:15486207
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This tab holds annotation information from the InterPro database.
InterPro entry IPR019653
The RegB endoribonuclease encoded by Bacteriophage T4 is a unique sequence-specific nuclease that cleaves in the middle of GGAG or, in a few cases, GGAU tetranucleotides, preferentially those found in the Shine-Dalgarno regions of early phage mRNAs. T4 regB expression is regulated autogenously by attacking its own mRNA. The deduced primary structure of RegB proteins in many phages is almost identical to that of T4, while the sequences of RegB encoded by Enterobacteria phage RB69, Enterobacteria phage TuIa and Enterobacteria phage RB49 show substantial divergence from their T4 counterpart [PUBMED:15486207]. In RB49 regB expression is regulated by both RegB and Escherichia coli endoribonuclease E.
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Curation and family details
|Author:||Mistry J, Coggill P|
|Number in seed:||14|
|Number in full:||136|
|Average length of the domain:||142.10 aa|
|Average identity of full alignment:||24 %|
|Average coverage of the sequence by the domain:||90.69 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||4|
|Download:||download the raw HMM for this family|
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