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0  structures 131  species 0  interactions 157  sequences 7  architectures

Family: Rax2 (PF12768)

Summary: Cortical protein marker for cell polarity

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Cortical protein marker for cell polarity Provide feedback

Diploid yeast cells repeatedly polarize and bud from their poles, due probably to the presence of highly stable membrane markers, and Rax2 is one such marker. It is inherited immutably at the cell cortex for multiple generations, and has a half-life exceeding several generations. The persistent inheritance of cortical protein markers would provide a means of coupling a cell's history with the future development of a precise morphogenetic form [1]. Both Rax1 and Rax2 localise to the distal pole as well as to the division site and they interact both with each other and with Bud8p and Bud9p in the establishment and/or maintenance of the cortical markers for bipolar budding [2]. thus Rax2 is likely to control cell polarity during vegetative growth, and in fission yeast this is done by regulating the localisation of for3p [3].

Literature references

  1. Chen T, Hiroko T, Chaudhuri A, Inose F, Lord M, Tanaka S, Chant J, Fujita A;, Science. 2000;290:1975-1978.: Multigenerational cortical inheritance of the Rax2 protein in orienting polarity and division in yeast. PUBMED:11110666 EPMC:11110666

  2. Kang PJ, Angerman E, Nakashima K, Pringle JR, Park HO;, Mol Biol Cell. 2004;15:5145-5157.: Interactions among Rax1p, Rax2p, Bud8p, and Bud9p in marking cortical sites for bipolar bud-site selection in yeast. PUBMED:15356260 EPMC:15356260

  3. Choi E, Lee K, Song K;, Mol Cells. 2006;22:146-153.: Function of rax2p in the polarized growth of fission yeast. PUBMED:17085965 EPMC:17085965


External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR024982

Diploid yeast cells repeatedly polarize and bud from their poles, due probably to the presence of highly stable membrane markers, and Rax2 is one such marker. It is inherited immutably at the cell cortex for multiple generations, and has a half-life exceeding several generations. The persistent inheritance of cortical protein markers would provide a means of coupling a cell's history with the future development of a precise morphogenetic form [PUBMED:11110666]. Both Rax1 and Rax2 localise to the distal pole as well as to the division site and they interact both with each other and with Bud8p and Bud9p in the establishment and/or maintenance of the cortical markers for bipolar budding [PUBMED:15356260]. Thus Rax2 is likely to control cell polarity during vegetative growth, and in fission yeast this is done by regulating the localisation of for3p [PUBMED:17085965].

Domain organisation

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Alignments

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

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(34)
Full
(157)
Representative proteomes NCBI
(169)
Meta
(15)
RP15
(45)
RP35
(77)
RP55
(109)
RP75
(124)
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  Seed
(34)
Full
(157)
Representative proteomes NCBI
(169)
Meta
(15)
RP15
(45)
RP35
(77)
RP55
(109)
RP75
(124)
Alignment:
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(34)
Full
(157)
Representative proteomes NCBI
(169)
Meta
(15)
RP15
(45)
RP35
(77)
RP55
(109)
RP75
(124)
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You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

External links

MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER3.

Pfam alignments:

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Curation and family details

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Curation View help on the curation process

Seed source: Pfam-B_2071 (release 24.0)
Previous IDs: none
Type: Family
Author: Wood V, Coggill P
Number in seed: 34
Number in full: 157
Average length of the domain: 251.50 aa
Average identity of full alignment: 25 %
Average coverage of the sequence by the domain: 23.26 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild --amino -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 25.6 24.9
Trusted cut-off 25.6 25.2
Noise cut-off 25.5 24.6
Model length: 281
Family (HMM) version: 2
Download: download the raw HMM for this family

Species distribution

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