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12  structures 30  species 0  interactions 35  sequences 1  architecture

Family: MLANA (PF14991)

Summary: Protein melan-A

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This is the Wikipedia entry entitled "MLANA". More...

MLANA Edit Wikipedia article

Melan-A

Rendering of a MHC protein complexed with a MLANA derived peptide (yellow), from PDB 2GUO
Available structures
PDB Ortholog search: PDBe, RCSB
Identifiers
Symbols MLANA; MART-1; MART1
External IDs OMIM605513 MGI108454 HomoloGene4026 GeneCards: MLANA Gene
RNA expression pattern
PBB GE MLANA 206426 at tn.png
PBB GE MLANA 206427 s at tn.png
More reference expression data
Orthologs
Species Human Mouse
Entrez 2315 77836
Ensembl ENSG00000120215 ENSMUSG00000024806
UniProt Q16655 Q2TA50
RefSeq (mRNA) NM_005511 NM_029993
RefSeq (protein) NP_005502 NP_084269
Location (UCSC) Chr 9:
5.89 – 5.91 Mb
Chr 19:
29.7 – 29.71 Mb
PubMed search [1] [2]

Protein melan-A also known as melanoma antigen recognized by T-cells 1 or MART-1 is a protein that in humans is encoded by the MLANA gene.[1] A fragment of the protein, usually consisting of the nine amino acids 27 to 35, is bound by MHC class I complexes which present it to T cells of the immune system. These complexes can be found on the surface of melanoma cells. Decameric peptides (26-35) are being investigated as cancer vaccines.

Discovery and nomenclature[edit]

The names MART-1 and Melan-A were coined by two groups of researchers who independently sequenced the gene for this antigen in 1994. Both names are currently in common use. Kawakami et al. at the National Cancer Institute coined the term MART-1, which stands for "melanoma antigen recognized by T-cells."[2] Coulie et al. of Belgium called the gene Melan-A, presumably an abbreviation for "melanocyte antigen."[3]

Clinical significance[edit]

MART-1/Melan-A is a protein antigen that is found on the surface of melanocytes. Antibodies against the antigen are used in the medical specialty of anatomic pathology in order to recognize cells of melanocytic differentiation, useful for the diagnosis of a melanoma. The same name is also used to refer to the gene which codes for the antigen.

The MART-1 / Melan-A antigen is specific for the melanocyte lineage, found in normal skin, the retina, and melanocytes, but not in other normal tissues. It is thus useful as a marker for melanocytic tumors (melanomas) with the caveat that it is normally found in benign nevi as well.

Structure[edit]

MART-1 / Melan-A is a putative 18 kDa transmembrane protein consisting of 118 amino acids. It has a single transmembrane domain.

Regulation[edit]

Its expression is regulated by the Microphthalmia-associated transcription factor.[4][5]

References[edit]

  1. ^ "Entrez Gene: MLANA melan-A". 
  2. ^ Kawakami Y, Eliyahu S, Delgado CH, Robbins PF, Rivoltini L, Topalian SL, Miki T, Rosenberg SA (April 1994). "Cloning of the gene coding for a shared human melanoma antigen recognized by autologous T cells infiltrating into tumor". Proc. Natl. Acad. Sci. U.S.A. 91 (9): 3515–9. doi:10.1073/pnas.91.9.3515. PMC 43610. PMID 8170938. 
  3. ^ Coulie PG, Brichard V, Van Pel A, Wölfel T, Schneider J, Traversari C, Mattei S, De Plaen E, Lurquin C, Szikora JP, Renauld JC, Boon T (July 1994). "A new gene coding for a differentiation antigen recognized by autologous cytolytic T lymphocytes on HLA-A2 melanomas". J. Exp. Med. 180 (1): 35–42. doi:10.1084/jem.180.1.35. PMC 2191574. PMID 8006593. 
  4. ^ Du J, Miller AJ, Widlund HR, Horstmann MA, Ramaswamy S, Fisher DE (2003). "MLANA/MART1 and SILV/PMEL17/GP100 are transcriptionally regulated by MITF in melanocytes and melanoma". Am. J. Pathol. 163 (1): 333–43. doi:10.1016/S0002-9440(10)63657-7. PMC 1868174. PMID 12819038. 
  5. ^ Hoek KS, Schlegel NC, Eichhoff OM, et al. (2008). "Novel MITF targets identified using a two-step DNA microarray strategy". Pigment Cell Melanoma Res. 21 (6): 665–76. doi:10.1111/j.1755-148X.2008.00505.x. PMID 19067971. 


Further reading[edit]


This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Protein melan-A Provide feedback

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Literature references

  1. Kawakami Y, Eliyahu S, Delgado CH, Robbins PF, Rivoltini L, Topalian SL, Miki T, Rosenberg SA;, Proc Natl Acad Sci U S A. 1994;91:3515-3519.: Cloning of the gene coding for a shared human melanoma antigen recognized by autologous T cells infiltrating into tumor. PUBMED:8170938 EPMC:8170938

  2. Coulie PG, Brichard V, Van Pel A, Wolfel T, Schneider J, Traversari C, Mattei S, De Plaen E, Lurquin C, Szikora JP, Renauld JC, Boon T;, J Exp Med. 1994;180:35-42.: A new gene coding for a differentiation antigen recognized by autologous cytolytic T lymphocytes on HLA-A2 melanomas. PUBMED:8006593 EPMC:8006593

  3. Giordano F, Bonetti C, Surace EM, Marigo V, Raposo G;, Hum Mol Genet. 2009;18:4530-4545.: The ocular albinism type 1 (OA1) G-protein-coupled receptor functions with MART-1 at early stages of melanogenesis to control melanosome identity and composition. PUBMED:19717472 EPMC:19717472


External database links

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Domain organisation

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Alignments

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  Seed
(5)
Full
(35)
Representative proteomes NCBI
(30)
Meta
(0)
RP15
(1)
RP35
(1)
RP55
(5)
RP75
(22)
Alignment:
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  Seed
(5)
Full
(35)
Representative proteomes NCBI
(30)
Meta
(0)
RP15
(1)
RP35
(1)
RP55
(5)
RP75
(22)
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Pfam alignments:

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Curation and family details

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This family is new in this Pfam release.

Seed source: Jackhmmer:Q16655
Previous IDs: none
Type: Family
Author: Bateman A
Number in seed: 5
Number in full: 35
Average length of the domain: 108.70 aa
Average identity of full alignment: 59 %
Average coverage of the sequence by the domain: 98.78 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 27.0 27.0
Trusted cut-off 81.8 81.7
Noise cut-off 23.8 23.0
Model length: 118
Family (HMM) version: 1
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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the MLANA domain has been found. There are 12 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.

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