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0  structures 88  species 0  interactions 212  sequences 5  architectures

Family: Rootletin (PF15035)

Summary: Ciliary rootlet component, centrosome cohesion

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This is the Wikipedia entry entitled "Rootletin ". More...

Rootletin Edit Wikipedia article

Ciliary rootlet coiled-coil, rootletin
External IDs MGI3529431 HomoloGene16811 GeneCards: CROCC Gene
Species Human Mouse
Entrez 9696 230872
Ensembl ENSG00000058453 ENSMUSG00000040860
UniProt Q5TZA2 Q8CJ40
RefSeq (mRNA) NM_014675 NM_001145958
RefSeq (protein) NP_055490 NP_001139430
Location (UCSC) Chr 1:
17.22 – 17.3 Mb
Chr 4:
141.02 – 141.06 Mb
PubMed search [1] [2]
Symbol Rootletin
Pfam PF15035

Rootletin also known as ciliary rootlet coiled-coil protein (CROCC) is a protein that in humans is encoded by the CROCC gene.[1][2][3] Rootletin is a component of the ciliary rootlet, and, together with CEP68 and CEP250, is required for centrosome cohesion.[4]

Rootletin is an important protein in the ciliary rootlet, particular for the structure and can be considered an important protein in mitosis as it is a centrosome linker.


This protein forms part of the ciliary rootlet structure. It also helps to contribute to the centrosome cohesion before mitosis.[5] Expression of rooletin leads to the formation of fibrous protein.


This protein is part of the structure of a ciliary rootlet. This cytoskeletal-like structure starts from the basal body at one end of the cilium and extends towards nucleus. Its molecular structure consists of a globular head domain and a tail domain made up of coiled-coil structures.[1]

Protein interactions[edit]

A large coiled-coil protein, C-Nap1, is a docking site for the fibrous tether to proximal ends of centrioles which Rootletin physically interacts with.Furthermore, Rootletin is phosphorylated by Nek2 kinase.[6]


  1. ^ a b Yang J, Liu X, Yue G, Adamian M, Bulgakov O, Li T (Nov 2002). "Rootletin, a novel coiled-coil protein, is a structural component of the ciliary rootlet". J Cell Biol 159 (3): 431–40. doi:10.1083/jcb.200207153. PMC 2173070. PMID 12427867. 
  2. ^ McClintock TS, Glasser CE, Bose SC, Bergman DA (Jan 2008). "Tissue expression patterns identify mouse cilia genes". Physiol Genomics 32 (2): 198–206. doi:10.1152/physiolgenomics.00128.2007. PMID 17971504. 
  3. ^ "Entrez Gene: CROCC ciliary rootlet coiled-coil, rootletin". 
  4. ^ Graser S, Stierhof YD, Nigg EA (December 2007). "Cep68 and Cep215 (Cdk5rap2) are required for centrosome cohesion". J. Cell. Sci. 120 (Pt 24): 4321–31. doi:10.1242/jcs.020248. PMID 18042621. 
  5. ^ Bahe S, Stierhof YD, Wilkinson CJ, Leiss F, Nigg EA (October 2005). "Rootletin forms centriole-associated filaments and functions in centrosome cohesion". J. Cell Biol. 171 (1): 27–33. doi:10.1083/jcb.200504107. PMC 2171225. PMID 16203858. 
  6. ^ Lim HH, Zhang T, Surana U (July 2009). "Regulation of centrosome separation in yeast and vertebrates: common threads". Trends Cell Biol. 19 (7): 325–33. doi:10.1016/j.tcb.2009.03.008. PMID 19576775. 

Further reading[edit]

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Ciliary rootlet component, centrosome cohesion Provide feedback

No Pfam abstract.

Literature references

  1. Fry AM, Mayor T, Meraldi P, Stierhof YD, Tanaka K, Nigg EA;, J Cell Biol. 1998;141:1563-1574.: C-Nap1, a novel centrosomal coiled-coil protein and candidate substrate of the cell cycle-regulated protein kinase Nek2. PUBMED:9647649 EPMC:9647649

  2. Helps NR, Luo X, Barker HM, Cohen PT;, Biochem J. 2000;349:509-518.: NIMA-related kinase 2 (Nek2), a cell-cycle-regulated protein kinase localized to centrosomes, is complexed to protein phosphatase 1. PUBMED:10880350 EPMC:10880350

  3. Mayor T, Hacker U, Stierhof YD, Nigg EA;, J Cell Sci. 2002;115:3275-3284.: The mechanism regulating the dissociation of the centrosomal protein C-Nap1 from mitotic spindle poles. PUBMED:12140259 EPMC:12140259

  4. Bahe S, Stierhof YD, Wilkinson CJ, Leiss F, Nigg EA;, J Cell Biol. 2005;171:27-33.: Rootletin forms centriole-associated filaments and functions in centrosome cohesion. PUBMED:16203858 EPMC:16203858

  5. Kim K, Lee S, Chang J, Rhee K;, Exp Cell Res. 2008;314:3692-3700.: A novel function of CEP135 as a platform protein of C-NAP1 for its centriolar localization. PUBMED:18851962 EPMC:18851962

  6. Mardin BR, Lange C, Baxter JE, Hardy T, Scholz SR, Fry AM, Schiebel E;, Nat Cell Biol. 2010;12:1166-1176.: Components of the Hippo pathway cooperate with Nek2 kinase to regulate centrosome disjunction. PUBMED:21076410 EPMC:21076410

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This family is new in this Pfam release.

Seed source: Jackhmmer:Q86T23
Previous IDs: none
Type: Family
Author: Coggill P
Number in seed: 29
Number in full: 212
Average length of the domain: 174.70 aa
Average identity of full alignment: 35 %
Average coverage of the sequence by the domain: 10.71 %

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build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 25.1 25.1
Trusted cut-off 25.4 25.3
Noise cut-off 25.0 25.0
Model length: 182
Family (HMM) version: 1
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