Summary: Ciliary rootlet component, centrosome cohesion
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Rootletin Edit Wikipedia article
|Ciliary rootlet coiled-coil, rootletin|
Rootletin also known as ciliary rootlet coiled-coil protein (CROCC) is a protein that in humans is encoded by the CROCC gene. Rootletin is a component of the ciliary rootlet, and, together with CEP68 and CEP250, is required for centrosome cohesion.
This protein forms part of the ciliary rootlet structure. It also helps to contribute to the centrosome cohesion before mitosis. Expression of rooletin leads to the formation of fibrous protein.
This protein is part of the structure of a ciliary rootlet. This cytoskeletal-like structure starts from the basal body at one end of the cilium and extends towards nucleus. Its molecular structure consists of a globular head domain and a tail domain made up of coiled-coil structures.
 Protein interactions
A large coiled-coil protein, C-Nap1, is a docking site for the fibrous tether to proximal ends of centrioles which Rootletin physically interacts with.Furthermore, Rootletin is phosphorylated by Nek2 kinase.
- Yang J, Liu X, Yue G, Adamian M, Bulgakov O, Li T (Nov 2002). "Rootletin, a novel coiled-coil protein, is a structural component of the ciliary rootlet". J Cell Biol 159 (3): 431–40. doi:10.1083/jcb.200207153. PMC 2173070. PMID 12427867. //www.ncbi.nlm.nih.gov/pmc/articles/PMC2173070/.
- McClintock TS, Glasser CE, Bose SC, Bergman DA (Jan 2008). "Tissue expression patterns identify mouse cilia genes". Physiol Genomics 32 (2): 198–206. doi:10.1152/physiolgenomics.00128.2007. PMID 17971504.
- "Entrez Gene: CROCC ciliary rootlet coiled-coil, rootletin". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=9696.
- Graser S, Stierhof YD, Nigg EA (December 2007). "Cep68 and Cep215 (Cdk5rap2) are required for centrosome cohesion". J. Cell. Sci. 120 (Pt 24): 4321–31. doi:10.1242/jcs.020248. PMID 18042621.
- Bahe S, Stierhof YD, Wilkinson CJ, Leiss F, Nigg EA (October 2005). "Rootletin forms centriole-associated filaments and functions in centrosome cohesion". J. Cell Biol. 171 (1): 27–33. doi:10.1083/jcb.200504107. PMC 2171225. PMID 16203858. //www.ncbi.nlm.nih.gov/pmc/articles/PMC2171225/.
- Lim HH, Zhang T, Surana U (July 2009). "Regulation of centrosome separation in yeast and vertebrates: common threads". Trends Cell Biol. 19 (7): 325–33. doi:10.1016/j.tcb.2009.03.008. PMID 19576775.
 Further reading
- Ching YP, Chan SF, Jeang KT, Jin DY (2006). "The retroviral oncoprotein Tax targets the coiled-coil centrosomal protein TAX1BP2 to induce centrosome overduplication.". Nat. Cell Biol. 8 (7): 717–24. doi:10.1038/ncb1432. PMID 16767081.
- Gregory SG, Barlow KF, McLay KE, et al. (2006). "The DNA sequence and biological annotation of human chromosome 1.". Nature 441 (7091): 315–21. doi:10.1038/nature04727. PMID 16710414.
- Andersen JS, Wilkinson CJ, Mayor T, et al. (2003). "Proteomic characterization of the human centrosome by protein correlation profiling.". Nature 426 (6966): 570–4. doi:10.1038/nature02166. PMID 14654843.
- Behrends U, Schneider I, Rössler S, et al. (2003). "Novel tumor antigens identified by autologous antibody screening of childhood medulloblastoma cDNA libraries.". Int. J. Cancer 106 (2): 244–51. doi:10.1002/ijc.11208. PMID 12800201.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. //www.ncbi.nlm.nih.gov/pmc/articles/PMC139241/.
- Seki N, Ohira M, Nagase T, et al. (1998). "Characterization of cDNA clones in size-fractionated cDNA libraries from human brain.". DNA Res. 4 (5): 345–9. doi:10.1093/dnares/4.5.345. PMID 9455484.
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Ciliary rootlet component, centrosome cohesion Provide feedback
No Pfam abstract.
Fry AM, Mayor T, Meraldi P, Stierhof YD, Tanaka K, Nigg EA;, J Cell Biol. 1998;141:1563-1574.: C-Nap1, a novel centrosomal coiled-coil protein and candidate substrate of the cell cycle-regulated protein kinase Nek2. PUBMED:9647649 EPMC:9647649
Helps NR, Luo X, Barker HM, Cohen PT;, Biochem J. 2000;349:509-518.: NIMA-related kinase 2 (Nek2), a cell-cycle-regulated protein kinase localized to centrosomes, is complexed to protein phosphatase 1. PUBMED:10880350 EPMC:10880350
Mayor T, Hacker U, Stierhof YD, Nigg EA;, J Cell Sci. 2002;115:3275-3284.: The mechanism regulating the dissociation of the centrosomal protein C-Nap1 from mitotic spindle poles. PUBMED:12140259 EPMC:12140259
Mardin BR, Lange C, Baxter JE, Hardy T, Scholz SR, Fry AM, Schiebel E;, Nat Cell Biol. 2010;12:1166-1176.: Components of the Hippo pathway cooperate with Nek2 kinase to regulate centrosome disjunction. PUBMED:21076410 EPMC:21076410
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|Number in seed:||29|
|Number in full:||212|
|Average length of the domain:||174.70 aa|
|Average identity of full alignment:||35 %|
|Average coverage of the sequence by the domain:||10.71 %|
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